Sequences from confirmation amplicon


Confirmation sequences for line SALK_126880c and hit At1g67070 are shown.
Line ID SALK_126880c
Locus At1g67070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25042190
Primers used for PCR 16ht - LBb3, show primer details
   
Sequence Name 23-K112995-SALK_126880C-16ht
GenBank Accession LN484949 [GenBank]
Sequencing primer gene specific primer, 16ht: 5'-GACTCGTTGTCCTGTATTAGCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K112995-SALK_126880C-16ht
GCCCATTCGTAGTTCTTCCAAACATCTCAGCCGTTGGATCTCTTCACCGCCGGTTAACTT
GGCTTGATCGTGACCATTCGTTTGGATTGCGTCTGCTCCCATTTTTGGCCGTTTATTTTA
TTTTTCCTTCGATGATGAATCTGATAATATAGAGTGATGGGGTTTTCTTGTAATCTATAT
ATAATGTATAAATGAAATATATTCCTTGTAAGAGGGTTAAAATTCAATCATATATAACAC
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCGAAATCGGCAAAA
   
Sequence Name 21-K112994-SALK_126880C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112994-SALK_126880C-R210
CGAAGGAAAAANAAAAAAAACGGCCAAAAAAGGGAGCAGACGCAAGCCAAACGAAAGGGC
ACGAGCAAGCCAAGAAAACCGGCGGAGAAGAGACCCAACGGCGGAGATGTGCCGCGAAGA
ACAACGAAGGGGGCAAACGCGGACCGGAGACAGGGGAGGCGAGACCCCAAGAGGCAAACA
CAGGACAACGA

Last Updated on 06.12.2018 16:11