Sequences from confirmation amplicon


Confirmation sequences for line SALK_126948c and hit At1g78510 are shown.
Line ID SALK_126948c
Locus At1g78510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29537058
Primers used for PCR 97by - LBb3, show primer details
   
Sequence Name 25-K108893-SALK_126948C-97by
GenBank Accession LN484996 [GenBank]
Sequencing primer gene specific primer, 97by: 5'-AAAAACTCGATCGGCTGATACTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K108893-SALK_126948C-97by
TTTTTTGTTGAATTTTTCCATACACCCAATTTCCCGTGGAAGAAAAGGAGTCAGCTTTTT
CGTTTGCTTTAAAGCTTGAATCTTTACATAGTTCCAGTTACTTTCTATAGAGCTTCATAA
TCCCTAGTTGCCCAAAAGGATTCTGGGTTTTGTGAGAATGATGACGTCATGTCGGAATAT
AGATTTAGGTACGATGATGATGGCATGTGGTTGTGGCCGTCGTCAGTTTCCTTCATTAGC
GAAGACTGTTTGTAAGTTTACTAACACATTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCAGCAAAA
   
Sequence Name 09-K108892-SALK_126948C-R210
GenBank Accession LN484514 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K108892-SALK_126948C-R210
GTCATGTGTTAGTAACTTACAAACAGTCTTCGCTAATGAAGGAAACTGACGACGGCCACT
ACCACATGCCATCATCATCGTACCTAAATCTATATTCCGACATGACGTCATCATTCTCAC
AAAACCCAGAATCCTTTTGGGCAACTAGGGATTATGAAGCTCTATAGAAAGTAACTGGAA
CTATGTAAAGATTCAAGCTTTAAAGCAAACGAAAAAGCTGACTCCTTTTCTTCCACGGGA
AATTGGGTGTATGGAAAAATTACAACAAAAAAAGGTACAAAAATACAAAATCAAGTGGGT
GATTAAGTATCAGCCGATCGAGTTTTT

Last Updated on 06.12.2018 16:11