Sequences from confirmation amplicon


Confirmation sequences for line SALK_127167c and hit At1g24150 are shown.
Line ID SALK_127167c
Locus At1g24150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8549581
Primers used for PCR 28di - LBb3, show primer details
   
Sequence Name 42-K110394-SALK_127167C-28di
GenBank Accession LN485329 [GenBank]
Sequencing primer gene specific primer, 28di: 5'-AGTCTTGACATGAGGAGGATGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K110394-SALK_127167C-28di
GATTACCTATGAGAAGTCGAAGATCTTCCTCTGAGAAGAGGAGTCTCCGTCACCGGTCCA
GATTTCTTCTTACTCTTCGAATAAATCACATTCTTCTCTTCATCGTCTCCGTGGATGATC
TCTTTCCTAAAGCTTCCACCTTTATTATCTCTTTGACTCTGTTGTAACTTTCTCCAATAC
AACACATCGAGACCATTCTCATCTAGAATCAAACCCTTTACGTTTCCGCCGAATCGAGTA
AACCCTTCACGAGCTAAAGCCGCTTCCGCCAACGGAGGAACCGGTGGTTGAAGGGTGTTG
TCAACGCCACCGACTCTGTTTCTCCTCCGCCTACATTTATGGACGAGGAAGAAGAACACG
GCTGCTACAAGTAAAGTGCTTGCTGCTGTTATAAGCACAGCCCTCAAGATATTTCCTCTG
TCTGAATCCGATGACGAAGATGATGGGTTTTGCGGCGGCGATAGAACCGGAGATTGAACT
GGAGGGGTGATTGTGTCATTTGGGAAAAATGTTTCGATGTTTCGAGGAGAATCGGATTGA
GAAAAGGATTGATTAGGGAAAAAGAGGATTAGGGTTAAGAAAACCCTTAATAACACATTG
CGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCC
CAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 34-K110393-SALK_127167C-R210
GenBank Accession LN485200 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K110393-SALK_127167C-R210
ACCCTATCCTCTTTTTCCCTAATCAATCCTTTTCTCATCCGATTCTCCTCGAAACATCGA
AACATTTTTCCCAAATGACACAATCACCCCTCCAGTTCAATCTCCGGTTCTATCGCCGCC
GCAAAACCCATCATCTTCGTCATCGGATTCAGACAGAGGAAATATCTTGAGGGCTGTGCT
TATAACAGCAGCAAGCACTTTACTTGTAGCAGCCGTGTTCTTCTTCCTCGTCCATAAATG
TAGGCGGAGGAGAAACAGAGTCGGTGGCGTTGACAACACCCTTCAACCACCGGTTCCTCC
GTTGGCGGAAGCGGCTTTAGCTCGTGAAGGGTTTACTCGATTCGGCGGAAACGTAAAGGG
TTTGATTCTAGATGAGAATGGTCTCGATGTGTTGTATTGGAGAAAGTTACAACAGAGTCA
AAGAGATAATAAAGGTGGAAGCTTTAGGAAAGAGATCATCCACGGAGACGATGAAGAGAA
GAATGTGATTTATTCGAAGAGTAAGAAGAAATCTGGACCGGTGACGGAGACTCCTCTTCT
CAGAGGAAGATCTTCGACTTCTCATAGTGTAATTCACAACGACAATTACCGAAATGCCAC
CACCACTCATCCTCCTCATGTCAAGAC

Last Updated on 06.12.2018 16:11