Sequences from confirmation amplicon


Confirmation sequences for line SALK_127263c and hit At3g24110 are shown.
Line ID SALK_127263c
Locus At3g24110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8705242
Primers used for PCR 28sm - LBb3, show primer details
   
Sequence Name 49-K116586-SALK_127263C-28sm
GenBank Accession LN485485 [GenBank]
Sequencing primer gene specific primer, 28sm: 5'-TGTTTCCTTTAACCTTTGCCTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116586-SALK_127263C-28sm
TTTAGGTACAACTATAAAGACATAAGAAAAAGATCTATTGATAATCTATAATAAACATTG
AAATGATGAAAGGGCCACCAAAGTTTAAGCCTTTAACTTTGATACTATTCGTAATGCCTT
TCTTCATTACTCTTTATGCTTTACTTTATCTACTTACGCCTTGTTCCTCTCATCCATCTT
TTCAACAAACATCAGAATTAACATGAGTGAGTTCTCATTTCTCGAAATTTCATTTGCTAT
GTTCTTATGGTTTGATTCATAGTTTTGAAATACAAAAAACCGTGTAGGAAACATGCTTTG
CTGCTGTGTTAACTCGGAGAACAACAAGAAGTATGCAGAGCTGGATGCGAAGCTTGCACG
GAAAATGGTGGAGAGCCGGAGTATTTATCCGGGACACAGGAGTTTAAAGTCTATGGATTC
AATAATCATGAAGTTTCCTAAGCTAAGAGAAGGATTAAGAAACACATTGCGGACGTTTAG
TTGCCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACAACGGCTC
TCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAGTGGTGATTTTGTGCCGA
GCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTGGCAGGATATATTGTGGTGTAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
C
   
Sequence Name 32-K116585-SALK_127263C-R210
GenBank Accession LN485172 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116585-SALK_127263C-R210
GTCATTTGTTACACCACAATATATCCTGCCACCAGCCAGCCAACAGCTCCCCGACCGGCA
GCTCGGCACAAAATCACCACTCGATACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGG
GAGAGCCGTTGTAAGGCGGCAGACTTTGCTCATGTTACCGATGCTATTCGGAAGAACGGC
AACTAAACGTCCGCAATGTGTTTCTTAATCCTTCTCTTAGCTTAGGAAACTTCATGATTA
TTGAATCCATAGACTTTAAACTCCTGTGTCCCGGATAAATACTCCGGCTCTCCACCATTT
TCCGTGCAAGCTTCGCATCCAGCTCTGCATACTTCTTGTTGTTCTCCGAGTTAACACAGC
AGCAAAGCATGTTTCCTACACGGTTTTTTGTATTTCAAAACTATGAATCAAACCATAAGA
ACATAGCAAATGAAATTTCGAGAAATGAGAACTCACTCATGTTAATTCTGATGTTTGTTG
AAAAGATGGATGAGAGGAACAAGGCGTAAGTAGATAAAGTAAAGCATAAAGAGTAATGAA
GAAAGGCATTACGAATAGTATCAAAGTTAAAGGCTTAAACTTTGGTGGCCCTTTCATCAT
TTCAATGTTTATTATAGATTATCAATAGATCTTTTTCTTATGTCTTTATAGTTTGTACCT
TTAAACCTTTCTCTTGAATACAAGAATAATTAGAGGCA

Last Updated on 06.12.2018 16:11