Sequences from confirmation amplicon


Confirmation sequences for line SALK_127430c and hit At2g20580 are shown.
Line ID SALK_127430c
Locus At2g20580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8862737
Primers used for PCR 63qz - LBb3, show primer details
   
Sequence Name 18-K115992-SALK_127430C-63qz
GenBank Accession LN484788 [GenBank]
Sequencing primer gene specific primer, 63qz: 5'-ATGAATTATGTCACTCAACCACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115992-SALK_127430C-63qz
CATAAGCACAAGAAAGAAGTGTCATATCACAATACTTTCTAATCTTCTCATTGAAGGTCT
TCGAAACTTCAGCTGTAGCCTCTACACTTTCCTGGTAGATATTGGAACAAGTTGATGTTG
GTCAAGCAATGCAAATAATGATATACGAACAAAGAGCGAGATTATGTAAAACACATTGCT
AACCTGTTTGCCCAGGTATAGAAGTCCAAGACCAAGTGGCAAGAAACGAGTGAGGGCATC
ACCCAGTTCTGCCTCACTTCGATCCATCAAAGCAAATATAATAGACTGGGCTACTTCTTC
GTTACAGGAACCAACATATATCATTCCCAAAGAAAGTGAAGCAAATGCAATCACATCAAG
AGGCGCTTTTGCATCATTCAGTATCGGGGACAATTTGTTTCGAATCTGTTGAAACAGTGT
ACGTGTTAAAGTTTTGTGAACACCCGAAACATTCAAGTACTGGTGACGCTTAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 09-K115991-SALK_127430C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115991-SALK_127430C-R210
TATTTTCAAAGACCGCATTAGAGAGGGATGTGATGAGACTCTTCTTT

Last Updated on 06.12.2018 16:11