Sequences from confirmation amplicon


Confirmation sequences for line SALK_127440c and hit MKP11 are shown.
Line ID SALK_127440c
Locus MKP11 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5700855
Primers used for PCR 38ea - LBb3, show primer details
   
Sequence Name 20-K111285-SALK_127440C-38ea
GenBank Accession LN484847 [GenBank]
Sequencing primer gene specific primer, 38ea: 5'-AGTCTGCTACCGCTGTCAATAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K111285-SALK_127440C-38ea
AAGATACTCAGAATTTAAACATCAACTTTGCCATCTCAAAGAGACTAGAGAAACTAACTC
CTAAGCAAGAATAAACGAAAACTGAATTGGATACTACTGATAGTAACACAAGATTAGAGG
GCTTACTTTGGACCAGTGCATCCCATTGTGGTTCCCAAACCTCCATTAAGCTTCAGCACA
ACAAGCTTGTCCAACAGATATTTGGTCTCGGAAGCATCTTCAATACGATAAAACAGAACA
AAATAATAATTCTTTAATGGTATGTAGAAAATGATCCAATGTAAAACTAGCATACAGAGA
AATAACAGATTTGTATCATTCAGAAAAATAAAATTACCTTCAGAGACGTTAGCCATTTTA
TCATAAGGAACAACAATTTCATCAGTAGGTGTCTGGATCTTGCTCCATTCAATGTGCTGG
GCTTCACCACTTCAAATTCACAAAACATTACAGAATTTAGAACTAATTGGTCATCTAAAT
GTAAACAACCTAATTTTCTAGATCTGACAACAGATCCGATTATCAGTCATTAGTAGATCG
TTTCAAAACTCAGAACAAGGTCCAAGAACAGAGCAAACAAACACTAACTGGTAAACTCAA
AACTCAAATTCAGAAAATCTATGACAAACTAAGAGCACACGAAAAAACTTTAAAGGCCAG
AAATTATTTGAGATTCAAGATAACAGCAGTCAAAATTCGATCAATACACATCCGAAGCGG
ACGTACGAACCTGAGGTAACGTGAAACGAGGTTGATGAATCCACTCTTCTCATTCTCGCT
GAAACAGAAGGAAACAAATCAGTCTAAACGATGAATAAGATTTACCAGATCCAGAATCTG
AGACGAAAGCTAATCGAGAGAGCGTAAAACCTCATCTCAGTAAGTCCATCGACGGCGGAT
TTGAGCTGAGGGAGCTTCTCGGTTGCGGTGGCAGCCATGGATGATTTCTATATATATGGT
GGTGTAAACAAATTGACGCTTAGACNACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTTCACGGCTG
GGCCC
   
Sequence Name 09-K111284-SALK_127440C-R210
GenBank Accession LN484518 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K111284-SALK_127440C-R210
TTTGTTTACCCCAATATATATAGAAATCATCCATGGCTGCCACCGCAACCGAGAAGCTCC
CTCAGCTCAAATCCGCCGTCGATGGACTTACTGAGATGAGGTTTTACGCTCTCTCGATTA
GCTTTCGTCTCAGATTCTGGATCTGGTAAATCTTATTCATCGTTTAGACTGATTTGTTTC
CTTCTGTTTCAGCGAGAATGAGAAGAGTGGATTCATCAACCTCGTTTCACGTTACCTCAG
GTTCGTACGTCCGCTTCGGATGTGTATTGATCGAATTTTGACTGCTGTTATCTTGAATCT
CAAATAATTTCTGGCCTTTAAAGTTTTTTCGTGTGCTCTTAGTTTGTCATAGATTTTCTG
AATTTGAGTTTTGAGTTTACCAGTTAGTGTTTGTTTGCTCTGTTCTTGGACCTTGTTCTG
AGTTTTGAAACGATCTACTAATGACTGATAATCGGATCTGTTGTCAGATCTAGAAAATTA
GGTTGTTTACATTTAGATGACCAATTAGTTCTAAATTCTGTAATGTTTTGTGAATTTGAA
GTGGTGAAGCCCAGCACATTGAATGGAGCAAGATCCAGACACCTACTGATGAAATTGTTG
TTCCTTATGATAAAATGGCTAACGTCTCTGAAGGTAATTTTATTTTTCTGAATGATACAA
ATCTGTTATTTCTCTGTATGCTAGTTTTACATTGGATCATTTTCTACATACCATTAAAGA
ATTATTATTTTGTTCTGTTTTATCGTATTGAAGATGCTTCCGAGACCAAATATCTGTTGG
ACAAGCTTGTTGTGCTGAAGCTTAATGGAGGTTTGGGAACCACAATGGGATGCACTGGTC
CAAAGTAAGCCCTCTAATCTTGTGTTACTATCAGTAGTATCCAATTCAGTTTTCGTTTAT
TCTTGCTTAGGAGTTAGTTTCTCTAGTCTCTTTGAGATGGCAAAGTTGATGTTTAAATTC
TGAGGTATCTTCCTCAGCCTGAAGATCTATCTTTCTTGGTCTATAGGATT

Last Updated on 06.12.2018 16:11