Sequences from confirmation amplicon


Confirmation sequences for line SALK_127790c and hit At5g07890 are shown.
Line ID SALK_127790c
Locus At5g07890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2519342
Primers used for PCR 31ty - LBb3, show primer details
   
Sequence Name 42-K117180-SALK_127790C-31ty
GenBank Accession LN485338 [GenBank]
Sequencing primer gene specific primer, 31ty: 5'-TGCTCGGATTTAGACATACACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117180-SALK_127790C-31ty
ATTCCTTGATTCGCCAATTTGAACTCAGATCGTGCAGATGTTCATTGAGATAATTCACTT
CCTTGCTTCTGAAAATTAGTTGATCCCGCAAGTAATCTACAAGGCGAAGATTAATATATG
CTGAAACATATTATTATTAGTTCAGAAAAATCAAGACTGGTTGGCTTAAATAGAAAATTA
CCAATTTCCTTATAGCAATTCAATAATTCTTTCTCCATCATCTGAATTCTTGCTGTGTCT
TCTAAGCGAGATTCAGATAATGACTTCGTGTGAAGTTCCAACCTCTGCGGTGAAGAGAAG
ATTAAGGTCCGAAAACTCAACCAACAGAATGAGAAAATACTTTCCCTGAATTGCTCATAT
TAAACAAGGAGAAGAAGACTACTCTGACTAGTTCAATACTATGGGGCTGTGATTCTCTCA
GCAAGTCCTTTTGTTTCCTTAGCTGCCAATAACAAATCAAAGAGCACAAAACAAAACAGA
AATCAACTTCAGCAATCTAAGAGTCAGAAACTACCTTATAATGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 21-K117179-SALK_127790C-R210
GenBank Accession LN484893 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117179-SALK_127790C-R210
GTAGTTTCTGACTCTTAGATTGCTGAAGTTGATTTCTGTTTTGTTTTGTGCTCTTTGATT
TGTTATTGGCAGCTAAGGAAACAAAAGGACTTGCTGAGAGAATCACAGCCCCATAGTATT
GAACTAGTCAGAGTAGTCTTCTTCTCCTTGTTTAATATGAGCAATTCAGGGAAAGTATTT
TCTCATTCTGTTGGTTGAGTTTTCGGACCTTAATCTTCTCTTCACCGCAGAGGTTGGAAC
TTCACACGAAGTCATTATCTGAATCTCGCTTAGAAGACACAGCAAGAATTCAGATGATGG
AGAAAGAATTATTGAATTGCTATAAGGAAATTGGTAATTTTCTATTTAAGCCAACCAGTC
TTGATTTTTCTGAACTAATAATAATATGTTTCAGCATATATTAATCTTCGCCTTGTAGAT
TACTTGCGGGATCAACTAATTTTCAGAAGCAAGGAAGTGAATTATCTCAATGAACATCTG
CACGATCTTGAGTTCAAATTGGCTGAATCAAGGAATCTGGAAGAAGAAGTTAACTCTTTA
AGAGATGAGTTGTGTATGTCTATTTTCCGAGCA

Last Updated on 06.12.2018 16:11