Sequences from confirmation amplicon


Confirmation sequences for line SALK_128365 and hit At3g02730 are shown.
Line ID SALK_128365
Locus At3g02730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:588612
Primers used for PCR 02ah - R204, show primer details
   
Sequence Name 78-K106742-SALK_128365C-R210
GenBank Accession LN486183 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K106742-SALK_128365C-R210
GTCATTTGTTTAATTTGTCCGGTTGCAGCGATTGAAACAGCGAGGTCTGCTGCTTCCGGA
TGAACCAGTCTCTCATGTCTTTACCATAATTGTTTGTATGTAATGTGAAATCTGGTGTTA
TCCCTATATATAGGTGATGAGGAAACGTCAGCAAAAGATTATCTTGTATGTGTAAAAGTT
TCAAGAAATATGATCATGAAAATGATTATCTTTAGGTTTCCAGTTGATTCGGTCTGAAGG
ATTTCTAAGCAATTCGGAACTGAGTTTGGAAGATTCGGTGCAATGTGCACTAGCAAGTTT
GTATTTTTAATTGGGGATATAGTTTGAAAGTTATGAATTACAATTGCGAGAACTAGCACA
AGTCTTATACAATTCATTCTTTACATAAAGCATCAACAGTGGGGTTCTTTATGAAACACA
ACTGATTGATTTGACAGTAAGCTAGTTAAAA
   
Sequence Name 94-K106743-SALK_128365C-02ah
GenBank Accession LN486356 [GenBank]
Sequencing primer gene specific primer, 02ah: 5'-GAGAAGAAGGCTGGTGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K106743-SALK_128365C-02ah
GTTCAGTAAGAAACCATGCCACACTTTTGCTTTAACTACTAACTTTGATGACCTAGTTTC
CTCGAGTTATGATGCTCTGGTCATTCCTGGAGGCAGAGCACCAGAATATCTGGCCTTGAA
CGAACATGTCCTTAACATAGTTAAAGAGTTCATGAATTCTGAGAAACCAGTTGCGTCTAT
TTGCCACGGACAACAGATCTTAGCTGCTGCTGGTGTCCTCAAGGTCAGCACTGTTCCTTC
GTAGCTCTAATTTTATTTAACTATGTCCTCCTTTTTCTTGCCACTACAAGTTGTTTGAGG
AATTTATTCTATGTGACCTGTTCACTGGCTGGTGCAGGGAAGGAAATGCACTGCTTATCC
AGCCGTGAAACTGAACGTGGTACTTGGAGGAGGAACTTGGCTAGAACCAGATCCAATCGA
CAGGTGCTTCACTGACGGGAATCTAGTAATTGGCGCATCATGGCCTGGACACCTGGAGTT
TGTTTCTCAACTAATGGCTTTGTTGGG
Primers used for PCR 03ah - LBb3, show primer details
   
Sequence Name 75-K118526-SALK_128365C-03ah
GenBank Accession LN486141 [GenBank]
Sequencing primer gene specific primer, 03ah: 5'-TCAAAGTTTCCGTGGCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K118526-SALK_128365C-03ah
TGTGGTTCATTTAGATTCCATAGTGAATGTTTTGGTTTTGATGTTGGTAGTCTTTAAAGA
GAGTTTCAATTCAAGATGTTCTATTCTTGGATACCTTATGCAGGTGTGGTCCATGTAAAG
TGATTGCCCCTAAATACAAAGCTTTATCAGAGAAGTACGACGACGTTGTGTTTCTAAAGC
TTGACTGCAATCCAGATAACCGGGTCTGAGTTCTGCTCTTTTATCTCTCTTCTTTTGGTA
ACTCGGTTTTCCTAAACCATATATCAATTGAACCATAGCTAGTAGCAGCTAAGGATAATC
AATACCTGTTTTTGTTGTTGCTACTACTACCTGTCTTTGTTGTTGCTGCTAAGCCATTGA
AACTCTTCATTGGTGCAGCCATTGGCAAAAGAGCTAGGAATAAGAGTGGTTCCAACTTTC
AAGATTTTGAAGGATAACAAGGTTGTCAAGGAAGTGACCGGTGCAAAATACGATGATCTG
CTGCGATTAAATAATATATTGCTGTTTAAACAAATTTGTTTAGACAACTTAGACAAATTG
ACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
G
   
Sequence Name 65-K118525-SALK_128365C-R210
GenBank Accession LN485884 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K118525-SALK_128365C-R210
GTCATTTGTCTAGTTGTCTAAACAAATTTGTTTAACAGCAATATATTATTTAATCGCAGC
AGATCATCGTATTTTGCACCGGTCACTTCCTTGACAACCTTGTTATCCTTCAAAATCTTG
AAAGTTGGAACCACTCTTATTCCTAGCTCTTTTGCCAATGGCTGCACCAATGAAGAGTTT
CAATGGCTTAGCAGCAACAACAAAGACAGGTAGTAGTAGCAACAACAAAAACAGGTATTG
ATTATCCTTAGCTGCTACTAGCTATGGTTCAATTGATATATGGTTTAGGAAAACCGAGTT
ACCAAAAGAAGAGAGATAAAAGAGCAGAACTCAGACCCGGTTATCTGGATTGCAGTCAAG
CTTTAGAAACACAACGTCGTCGTACTTCTCTGATAAAGCTTTGTATTTAGGGGCAATCAC
TTTACATGGACCACACCTGCATAAGGTATCCAAGAATAGAACATCTTGAATTGAAACTCT
CTTTAAAGACTACCAACATCAAAACCAAAACATTCACTATGGAATCTAAATGAACCACAT
CTGCAGAAGAATCTAACTGAGAAGATTTTTCAATCTTAGTTGAAAGCCACGAAAACTTTG
Primers used for PCR CG68 - LBb3, show primer details
   
Sequence Name 90-K118524-SALK_128365C-CG68
GenBank Accession LN486336 [GenBank]
Sequencing primer gene specific primer, CG68: 5'-TACGACGACGTTGTGTTTCTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K118524-SALK_128365C-CG68
GCTCTTTATCTCTCTTCTTTTGGAACTCGGTTTTCCTAAACCATATATCAATTGAACCAT
AGCTAGTAGCAGCTAAGGATAATCAATACCTGTTTTTGTTGTTGCTACTACTACCTGTCT
TTGTTGTTGCTGCTAAGCCATTGAAACTCTTCATTGGTGCAGCCATTGGCAAAAGAGCTA
GGAATAAGAGTGGTTCCAACTTTCAAGATTTTGAAGGATAACAAGGTTGTCAAGGAAGTG
ACCGGTGCAAAATACGATGATCTGCTGCGATTAAATAATATATTGCTGTTTAAACAAATT
TGTTTAGACAACTTAGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 89-K118523-SALK_128365C-R210
GenBank Accession LN486329 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K118523-SALK_128365C-R210
TTTGTCTAGTTGTCTAAACAAATTTGTTTAAACAGCAATATATTATTTAATCGCAGCAGA
TCATCGTATTTTGCACCGGTCACTTCCTTGACAACCTTGTTATCCTTCAAAATCTTGAAA
GTTGGAACCACTCTTATTCCTAGCTCTTTTGCCAATGGCTGCACCAATGAAGAGTTTCAA
TGGCTTAGCAGCAACAACAAAGACAGGTAGTAGTAGCAACAACAAAAACAGGTATTGATT
ATCCTTAGCTGCTACTAGCTATGGTTCAATTGATATATGGTTTAGGAAAACCGAGTTACC
AAAAGAAGAGAGATAAAAGAGCAGAACTCAGACCCGGTTATCTGGATTGCAGTCAAGCTT
TAGAAACACACGTTCGTCGT

Last Updated on 06.12.2018 16:11