Sequences from confirmation amplicon


Confirmation sequences for line SALK_128723c and hit At1g05620 are shown.
Line ID SALK_128723c
Locus At1g05620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1680838
Primers used for PCR 27rb - LBb3, show primer details
   
Sequence Name 48-K116274-SALK_128723C-27rb
GenBank Accession LN485460 [GenBank]
Sequencing primer gene specific primer, 27rb: 5'-AGACTTTCTCATCCGCAATTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116274-SALK_128723C-27rb
CCTTGATAATGCAGGCTGTTCAGCTCGATCACAGAGTTTTCGAAAAATGTCGGACAAATT
GTTCTTCTTGGTGGAGCATTTGCAGTAAACGGAAATGTAAATCCAGCTTCGGAAGCTAAT
GTAAGTTCCTTCACCCGTTAAAGTAATGTGATATTCATACACATAACACATCACTTTCTA
TCAAAAACTCTTCTTTTAGTTTTCACATCTGTTTAACCAATTTTTTGGTTAGGGTAGATT
TTTGGCGATCCCGAAGCTGCAGATATTGTGTTCACATGTGGGGCCGATATAATTGCTGTG
GGAATCAATGTGACCCATCAAGTTATTATGACAGGTTATCTTGGATAATCTTTTTTCCTT
ATTTGATGTTTTATATTGAAAATATACAAATGCTGTCAACAGAACCGTGATGATCCTGAT
TGTTGCCTTTATGGCTCGAGGTTTTCATTACTTAATAACACAAATTAAGCGTCAATATAT
TGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATC
CTGTTTGATGGTGG
   
Sequence Name 46-K116273-SALK_128723C-R210
GenBank Accession LN485418 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K116273-SALK_128723C-R210
ACGCTTAATTCGTGTTATTAAGTAATGAAAACCTCGAGCCATAAAGGCAACAATCAGGAT
CATCACGGTTCTGTTGACAGCATTTGTATATTTTCAATATAAAACATCAAATAAGGAAAA
AAGATTATCCAAGATAACCTGTCATAATAACTTGATGGGTCACATTGATTCCCACAGCAA
TTATATCGGCCCCACATGTGAACACAATATCTGCAGCTTCGGGATCGCCAAAAATCTACC
CTAACCAAAAAATTGGTTAAACAGATGTGAAAACTAAAAGAAGAGTTTTTGATAGAAAGT
GATGTGTTATGTGTATGAATATCACATTACTTTAACGGGTGAAGGAACTTACATTAGCTT
CCGAAGCTGGATTTACATTTCCGTTTACTGCAAATGCTCCACCAAGAAGAACAATTTGTC
CGACATTTTTCGAAAACTCTGGATCAAGCTGAACAGCCTGCATTATCAAGGTAATGAAAT
CTCCGATAAGATGATAGGAACAGAGATAAGTAAGAACCGGAATATAAAAATT

Last Updated on 06.12.2018 16:11