Sequences from confirmation amplicon


Confirmation sequences for line SALK_129744c and hit At5g10060 are shown.
Line ID SALK_129744c
Locus At5g10060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3146949
Primers used for PCR 65vi - LBb3, show primer details
   
Sequence Name 69-K117945-SALK_129744C-65vi
GenBank Accession LN485989 [GenBank]
Sequencing primer gene specific primer, 65vi: 5'-GGATAGACCAGAAGTCTTTGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K117945-SALK_129744C-65vi
TATGTGATGAGGTTGACGCTGTGAGCATTGCAGCAATAGATGCAGGCGTCATTTTTGATG
CTTGACCAGATTTGGTGTTGTCATTTGTCTCGGTTATCGTAGTGGCAGCAGTTGTTTGTT
TTGATGTGTAATCCTCATCATTAAGCCGTTTCTGCATGTTTTGGGCTTCCTCTGTTTGTT
CTTTAGCTACCTAATAATAACAAAATGGTTCATTAGCTGAAAGCTCAGGTTGGTCACAAA
ACGAAGATGCAGCGGGAATTCAAACACATACCTGAATCTGAGCTTTAAGATTGTCCAGTT
CGGATTCCTGACACAAGTAAGCAAAAGAATGAAAATTAGATTACCGAAATAGTGAACTTG
CTAAGTGCAAGAAAAGTATTTTGTACCTGTTCACGTAGTGCATCTTTAAGCTGGTTCACA
AGGGAACTTCTACTTCCTTGAACAGATTTAAGTTTCTCAATACATTGTCTCAAAAGGTAT
TCTTCTTCTTCCAATTCTTTCGCCAAAGATTTTCTCTTCGGGTTGTCTTTTGCTGTAGAG
ACACAAGTTGATTTACACAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 34-K117944-SALK_129744C-R210
GenBank Accession LN485209 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117944-SALK_129744C-R210
GTCATTTGTTGTGTAATCACTTGTGTCTCTACAGCAAAAGACAACCCGAAGAGAAAATCT
TTGGCGAAAGAATTGGAAGAAGAAGAATACCTTTTGAGACAATGTATTGAGAAACTTAAA
TCTGTTCAAGGAAGTAGAAGTTCCCTTGTGAACCAGCTTAAAGATGCACTACGTGAACAG
GTACAAAATACTTTTCTTGCACTTAGCAAGTTCACTATTTCGGTAATCTAATTTTCATTC
TTTTGCTTACTTGTGTCAGGAATCCGAACTGGACAATCTTAAAGCTCAGATTCAGGTATG
TGTTTGAATTCCCGCTGCATCTTCGTTTTGTGACCAACCTGAGCTTTCAGCTAATGAACC
ATTTTGTTATTATTAGGTAGCTAAAGAACAAACAGAGGAAGCCCAAAACATGCAGAAACG
GCTTAATGATGAGGATTACACATCAAAACAAACAACTGCTGCCACTACGATAACCGAGAC
AAATGACAACACCAAATCTGGTCAAGCATCAAAAATGACGCCTGCATCTATTGCTGCAAT
GCTCACAGCGTCAACCTCATCACATATGATAATGCAGTCTGTTCTATCTTCATTCGCAGC
TGAAGCAACAAAGACTTCGGGTCTATCC

Last Updated on 06.12.2018 16:11