Sequences from confirmation amplicon


Confirmation sequences for line SALK_129978c and hit At2g40820 are shown.
Line ID SALK_129978c
Locus At2g40820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17038321
Primers used for PCR 27md - LBb3, show primer details
   
Sequence Name 14-K114334-SALK_129978C-27md
GenBank Accession LN484673 [GenBank]
Sequencing primer gene specific primer, 27md: 5'-AACAATGTCTGCTTGTATCACTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114334-SALK_129978C-27md
GATTGGAAAATGTGAACTGCTCAGTGCTCAAGCTAAAAGCTCATGTCCTATAAGTTATTT
TGAGAACATAATAAGATTTGCCAAATGATCAGGGAAAGAGAGAATGCGAATTACCAGATA
TAGCTTCTCTTCGACTAGACTCAGATGCTGCACGGTACCAATGGATTGAGCATTTGGAAA
TATCTATACCACTGTCTGAGGAGGCACGGATTCTTAGGCACGAGCCAAGACTTTGTGCAC
CATCCAGAACATACGGACATGACTTGGACCCCTCAGCTTTCTTAATTATTGCTATCTATA
TGGATATTTGCAATTTTTAGAGTCAAGACAGTTTGTTGATGAAAGAATAACAAGAAGTAA
ACAAGACACAAAACCTACCTCTCTTTGAAGTTTAACGGAATATGCAGACTTCTCTTGGAT
TTGACTCTTTAAATCACGAAGCTCATGTTGCATACCGATCACCTAGATTATAAACCATCA
NAATCAGAAGTTTTGTTTGAACATTTTGGATTCCATTGTCAAAAAGATGACTAATGAAAG
AGATAGAAAGTCTTATAATGGAAAATTTATGTAGAGCACAGAAATGGAGTTGGACCATGG
TTGGTTTATGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAG
AGAGTTGCAGCANGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
   
Sequence Name 04-K114333-SALK_129978C-R210
GenBank Accession LN484375 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114333-SALK_129978C-R210
GTCCACTCCATTTCTGTGCTCTACATAAATTTTCCATTATAAGACTTTCTATCTCTTTCA
TTAGTCATCTTTTTGACAATGGAATCCAAAATGTTCAAACAAAACTTCTGATTTTGATGG
TTTATAATCTAGGTGATCGGTATGCAACATGAGCTTCGTGATTTAAAGAGTCAAATCCAA
GAGAAGTCTGCATATTCCGTTAAACTTCAAAGAGAGGTAGGTTTTGTGTCTTGTTTACTT
CTTGTTATTCTTTCATCAACAAACTGTCTTGACTCTAAAAATTGCAAATATCCATATAGA
TAGCAATAATTAAGAAAGCTGAGGGGTCCAAGTCATGTCCGTATGTTCTGGATGGTGCAC
AAAGTCTTGGCTCGTGCCTAAGAATCCGTGCCTCCTCAGACAGTGGTATAGATATTTCCA
AATGCTCAATCCATTGGTACCGTGCAGCATCTGAGTCTAGTCGAAGAGAAGCTATATCTG
GTAATTCGCATTCTCTCTTTCCCTGATCATTTGGCAAATCTTATTATGTTCTCAAAATAA
CTTATAGGACATGAGCTTTTAGCTTGAGCACTGAGCAGTTCACATTTTTCCAATCTTAGG
TGCCAACCGGTCAGTATATGCTCCAGAACCATTTGATGTCGGGCGAGTGATACAAGCAGA

Last Updated on 06.12.2018 16:11