Sequences from confirmation amplicon


Confirmation sequences for line SALK_132560c and hit At5g56030 are shown.
Line ID SALK_132560c
Locus At5g56030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22688710
Primers used for PCR 32hd - LBb3, show primer details
   
Sequence Name 44-K112623-SALK_132560C-32hd
GenBank Accession LN485374 [GenBank]
Sequencing primer gene specific primer, 32hd: 5'-GAAACAATAACCTTCTCGACCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K112623-SALK_132560C-32hd
TTCAACTTCTCCTTGAGCTCTTCTTTCTTCTTCTTTTCATCTTCGGTCTCATCCAGTTTC
AGACCTTCCTTGGTTGCAGAAACAAGTTTCTTTCCCTCGAATTCCTTAAGCTGACCAATA
GCGTACTCATCAATCGCATCAACCATGTAGAGAACTTCAATACCTTTCTTCTTGAGCTTC
TCAAGGAATGGAGAGTTCTCAACAGCCTTCTTGCTCTCACCAGTGATGTAGAAGATATCG
TTCTGACAAAAGTACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCCGAATCGAGCAAAA
   
Sequence Name 20-K112622-SALK_132560C-R210
GenBank Accession LN484850 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K112622-SALK_132560C-R210
TTTTGTCGAACGATATCTTCTACATCACTGGTGAGAGCAAGAAGGCTGTTGAGAACTCTC
CATTCCTTGAGAAGCTCAAGAAGAAAGGTATTGAAGTTCTCTACATGGTTGATGCGATTG
ATGAGTACGCTATTGGTCAGCTTAAGGAATTCGAGGGAAAGAAACTTGTTTCTGCAACCA
AGGAAGGTCTGAAACTGGATGAGACCGAAGATGAAAAGAAGAAGAAAGAAGAGCTCAAGG
AGAAGTTTGAAGGACTCTGCAAAGTGATCAAAGACGTTTTGGGTGACAAGGTCGAGAAGG
TTATTGTTT

Last Updated on 06.12.2018 16:11