Sequences from confirmation amplicon


Confirmation sequences for line SALK_133523c and hit At5g40650 are shown.
Line ID SALK_133523c
Locus At5g40650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16283256
Primers used for PCR 99by - LBb3, show primer details
   
Sequence Name 23-K109252-SALK_133523C-99by
GenBank Accession LN484941 [GenBank]
Sequencing primer gene specific primer, 99by: 5'-CTCATCAGCTACAGTGTTTCTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K109252-SALK_133523C-99by
TCTCTCTTTTCCTCACCATTAAAAAACAGCTTATCTGTTTGTAGATTAGTTATTTACTTT
CCAAGTTGCAAGAGGTATGTGTAAACATTTTTCTCTCTTATTCACCAATAAAGCAAAACT
CGAAATCCAAATGTTTGTATTAAGGCAAAGCTGAATGAGTGGATGATAAAAAAAGCAGAG
TTTTATTGCTTGTTGTAGGCAAAAAAAATTCATCAAGATATACCACAAAATAATCTAATT
CTGATAGTTGTGACAGCAATACAAGAATGAATCCAAAATTGGAGTTTCTTGCAGATTATT
AGAATCAAAAATCCATTAGGTACTGCCTGATTCATTTGTTGTTCAACCAGATTTCTGAAG
CTGCTTGATGTGTGTGATCTGTTTCCCTGGGTTCAGACAACTTAATAACACATTGCGGAC
GTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGC
CCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCA
GGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAATA
   
Sequence Name 33-K109255-SALK_133523C-R210
GenBank Accession LN485183 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K109255-SALK_133523C-R210
GGAACAGATCACCACATCAAGCAGCTTCGAAATCTGGTTGAACAACAAATGAATCAGGCA
GTACCTAATGGATTTTTGATTCTAATAATCTGCAAGAAACTCCAATTTTGGATTCATTCT
TGTATTGCTGTCACAACTATCAGAATTAGATTATTTTGTGGTATATCTTGATGAATTTTT
TTTGCCTACAACAAGCAATAAAACTCTGCTTTTTTTATCATCCACTCATTCAGCTTTGCC
TTAATACAAACATTTGGATTTCGAGTTTTGCTTTATTGGTGAATAAGAGAGAAAAATGTT
TACACATACCTCTTGCAACTTGGAAAGTAAATAACTAATCTACAAACAGATAAGCTTGTT
TTTTAATGTGTTGAGGAAAATGAGAGACTAATGATCAGTTTTCACGGGATAGAGCAAGAA
ACACTGTAGCTGATGAG

Last Updated on 06.12.2018 16:11