Sequences from confirmation amplicon


Confirmation sequences for line SALK_133989 and hit At2g17240 are shown.
Line ID SALK_133989
Locus At2g17240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7498546
Primers used for PCR BI62 - R204, show primer details
   
Sequence Name 58-K106282-SALK_133989C-R210
GenBank Accession LN485678 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K106282-SALK_133989C-R210
GCGTCATTTGTTTATAATGTAGAACAAATCACAAACTCTAAACCAAAACCTATTTATTCT
CAATCGGAGCTATTACCACAACGAATAGAAATGTGAAACTGGAAATTAAAAGATCCGAAA
TAAGACCCGACCCGACCCGGGGGAACTTACAATTGGGAAATCGAGAGGATTACGACGAGT
GAGCTTTTCTTCCTCAGGAGCCATAGACACAACTTGCAGCTGATTCCGATTGCGACGCGA
AGAAGAACGGTGAACGGAAGAGAAAGCAAAGACACGCGACGAAGAGCAACCAAGCGAGCG
CGAGTCGGAGGATATCTTCGTCTTCGTAGATAAAATATCGAGCGATGGAGAAGGAAGGAA
CCTCGCAGAGCAAAGCGACGCCNTGGTTCTCCGGGACGGAGCTAAATCTATTATCCACCA
ACCAAGAATTATCGTGACAGGTTCTAAGAAAGTTTTATGCTGCTGCGTTTGCGTTTTGAT
AGTGGGCTTTTAAAGGCTTTTATTGGGTGGCCCATAAATACTAAGCTTTGCGGACAATAG
CTAGGGCCTATGACTAACCACCTTGTTCATTCTTTCTAATCACTTTCCTTCACCATTATT
GTTTTGAAAATCTTCTTCTGACTTACGTTTTTTTATCTTTAAAATTTTTAACCACTCTTC
GTATTTTTTTTTTTAATTTTGTAGATAAAATATCGATATCACAACCAACAAATAAAGTTA
CTATGCACAATATATGATGCTAATTGTGAAAATTTGTAAACCACATTCTGTGCATAGTGA
TTTTCCAAAAAAAATCTTGAAAATTAGACTAGTTAGCTAATTAGTCCATAAGACTAACGA
GTGTATGTGTGGCACAGTAACAAAA
   
Sequence Name 74-K106283-SALK_133989C-BI62
GenBank Accession LN486096 [GenBank]
Sequencing primer gene specific primer, BI62: 5'-TAGTTAATCAGAAATTGGCACACG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K106283-SALK_133989C-BI62
TGGGGATAGTAGGCCAGCTACTTGGTTCCTTGTTAACACGAACCGGTGATCCGAGTTTTG
TTACTGTGCCACACATACACCTCGTCAGTCTTATGGACTAATTAGCTAACTAGTCTAATT
TTCAAGATTTTTTTTGGAAAATCACTATGCACAGAATGTGGTTTACAAATTTTCACAATT
AGCATCATATATTGTGCATAGTAACTTTATTTGTTGGTTGTGATATCGATATTTTATCTA
CAAAATTAAAAAAAAAAATACAAAAAGTGGTTAAAAATTTTAAAGATAAAAAAACGTAAG
TCAAAAAAAAATTTTCAAAACAATAATGGTGAAGGAAAGTGATTAAAAAGAATGAACAAG
GTGGTTAGTCATAGGCCCTAGCTATTGTCCGCAAAGCTTAGTATTTATGGGCCACCCAAT
AAAAGCCTTTAAAAGCCCACTATCAAAACGCAAACGCAGCACCATAAAACTTTCTTAAAA
CCTGTCACGATAATTCTGGGTTGGTGGATAATAAATTTAGCTCCGTCCCGGAAAACCATG
GCGTCGCTTTGCTCTGCGAGGTTCCTTCCTTCTCCATCGCTCGATATTTTATCTACAAAA
ACAAAAATTTCCTCCAACTCGCGCTCGCTTGGTTGCTCTTCGTCGCGTGTCTTTGCTTTC
TCTTCCGTTCACCGTTCTTCTTCGCGTCGCATTCGGAATCAGCTGCAAGTTGTGTCTATG
GCTCCGGAGGAAGAAAAGCTCACTCGTCGTAATCCTCTCGATTTCCCAATTGTAAGTTCC
CCCGGGTCGGGTCGGGTCTTATTTCGGATCTTTTAATTTCCAGTTTCACATTTC

Last Updated on 06.12.2018 16:11