Sequences from confirmation amplicon


Confirmation sequences for line SALK_134125c and hit At5g06910 are shown.
Line ID SALK_134125c
Locus At5g06910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2140467
Primers used for PCR 13gj - LBb3, show primer details
   
Sequence Name 28-K112064-SALK_134125C-13gj
GenBank Accession LN485084 [GenBank]
Sequencing primer gene specific primer, 13gj: 5'-TTTCTTATTTCCTGTGAAGTGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K112064-SALK_134125C-13gj
ACTTTGATAGTGACGAGAGATATTAGTTAGTTTCTATTGATTTCTGTCTTTTTAGCTAAT
CGAATCATCCTGATACCAAAATCCTAATCAAATATAAGATCACTGATCAAAACCTATGAT
TAGTGTTTAATCTACATAAAAATTTATATATCAAAACACCAAATTTTATGAAAGTAGACA
AATTCCAAACTATTTTCTAGAATCATGATAAACCAAACCCAGAAAACAAGCTTAAATTGA
AATCCCTAATAAAACCCTAAGTACGCGAGAGAAAGAGCAGCGATCGGAAAGAGAAATCCG
AAAATACCTCGTAAAGACTTGTCTCAGAGGATGGGCCAGCATTATCCATCTCAATCTCAT
CTTCTTCGGTTGTCGAAGCCCTAGATTTCTTTTTCCTACCCATCTCTCTGAACCCTAATT
AATTCGATAATATGGAATAACTGGGCTCTGTTGATTGGAGTCGGCGACTTAAAATGCTTC
ACAGAAGAAGATTATCTGGGCGCCAATATTTCCGTGTTCCCCGCCATTGTAGTGTAAACA
ATAATCCGATTATTCCGATTATCACAGGATATATTGTGGTGTAAACAAATTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 12-K112049-SALK_134125C-R210
GenBank Accession LN484611 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K112049-SALK_134125C-R210
CGTCATTTGTTTACCACAATATATCCTGTGATAATCGGAATAATCGGATTATTGTTTACC
TACAATGGCGGGGAACACGGAAATATTGGCGCCCAGATAATCTTCTTCTGTGAAGCATTT
TAAGTCGCCGACTCCAATCAACAGAGCCCAGTTATTCCATATTATCGAATTAATTAGGGT
TCAGAGAGATGGGTAGGAAAAAGAAATCTAGGGCTTCGACAACCGAAGAAGATGAGATTG
AGATGGATAATGCTGGCCCATCCTCTGAGACAAGTCTTTACGAGGTATTTTCGGATTTCT
CTTTCCGATCGCTGCTCTTTCTCTCGCGTACTTAGGGTTTTATTAGGGATTTCAATTTAA
GCTTGTTTTCTGGGTTTGGTTTATCATGATTCTAGAAAATAGTTTGGAATTTGTCTACTT
TCATAAAATTTGGTGTTTTGATATATAAATTTTTATGTAGATTAAACACTAATCATAGGT
TTTGATCAGTGATCTTATATTTGATTAGGATTTTGGTATCAGGATGATTCGATTAGCTAA
AAAGACAGAAATCAATAGAAACTAACTAATATCTCTCTGTCACTTTACTCAAAGGTTCTT
GGAGTTGAAAGAAGAGCCACTTCA

Last Updated on 06.12.2018 16:11