Sequences from confirmation amplicon


Confirmation sequences for line SALK_134251c and hit At1g02660 are shown.
Line ID SALK_134251c
Locus At1g02660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:572461
Primers used for PCR 28md - LBb3, show primer details
   
Sequence Name 15-K114334-SALK_134251C-28md
GenBank Accession LN484700 [GenBank]
Sequencing primer gene specific primer, 28md: 5'-CTGCAGAATTCTTGTGGTTAATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114334-SALK_134251C-28md
GCGAGCTTCTGATTCTTCACCAGATGAGACATTCTCCCCCGGGCATGAACTTCTTCCTTC
CGGAAACGGTTTATACCTTCTAACTAGTGATTTTGAATCGCCGGATATTGAAGATTCGGA
TGAGGAGCGGTTAAGAGCCGCGCAGACGGTTTTCTTGAACACCCCGCATCCTCTCGACAT
TCTCAGCGACAGATCGGCTTATGGGTCCAGCGGAACAATCCAAAGAGACCATGATATGAA
CTCGTATCTGAAAGCGGTTAGGAGTGTAATAAGAAATTACACATTGCGGTGTAAACAAAT
TGACAAATTGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCAAA
   
Sequence Name 05-K114333-SALK_134251C-R210
GenBank Accession LN484405 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114333-SALK_134251C-R210
CCGCAATGTGTAATTTCTTATTACACTCCTAACCGCTTTCAGATACGAGTTCATATCATG
GTCTCTTTGGATTGTTCCGCTGGACCCATAAGCCGATCTGTCGCTGAGAATGTCGAGAGG
ATGCGGGGTGTTCAAGAAAACCGTCTGCGCGGCTCTTAACCGCTCCTCATCCGAATCTTC
AATATCCGGCGATTCAAAATCACTAGTTAGAAGGTATAAACCGTTTCCGGAAGGAAGAAG
TTCATGCCCGGGGGAGAATGTCTCATCTGGTTGAAGAATCAGAAGCTCGCCCATCGGAGA
ATACAACATACTCTGCAGGAAAATGTCATTAACCACAAGAATTCTGCAG

Last Updated on 06.12.2018 16:11