Sequences from confirmation amplicon


Confirmation sequences for line SALK_135222c and hit At3g12060 are shown.
Line ID SALK_135222c
Locus At3g12060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3843586
Primers used for PCR 04jd - LBb3, show primer details
   
Sequence Name 33-K113259-SALK_135222C-04jd
GenBank Accession LN485187 [GenBank]
Sequencing primer gene specific primer, 04jd: 5'-TCCAAATGTACCAACAAAACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K113259-SALK_135222C-04jd
TCATATTCCGCGTCCAAACAGTGGCATTCTCGTAGTTGTTTCGAAAGACAAGGGGCATAA
TATAAAGAGAAATACCTCAAACTTTAGCGACGATTCCTAAGCAAATGTCACTTTACCGAA
GAAACACATCTCTCTCTCTCAAGTTTCTAGAAAAAGAGATTTGTTGACGGAGACAAGACT
CTTTTCTTTTTCACACTCTCGCTAAAAGACCCATTTTTTAATCTCTCTGAACTCTTAAAA
AAAAAAAAAACCCCAAACCCAAAGGTCAAAAAAAAAATTTCCCCCGCCCAAAAAAACCCC
CCCCCCGGGGGGGGGGACCCCTTAAAATTTTGCCTTTTTCGGGGGGTCCCCCCTTTCCCC
CCGGCCCCGGGAAAAAAAAAAATTTTTTTCCCCCCGGGAAACCCAAAAAAAATTTAAATT
TTCTTCCCCGTTTTGGAAAAAATTTTGGGGGCCTGGGGGGTTTTTTAACCTTTTAGCCTT
TCCCCAATAAAAGGGGCCCCCCCCCCTCCATTTTTTCCAAGGGTCCCCCCAAAAACCAGG
GGCCGAAATTCTCCAAATTTTTCCCGGGGAAGTTTGATTTTTATTTTCCCCAATTGGGGA
ACCCGGGCCCGGAAAAATCCCCGGGGATTTACAAAAAATTAACCTTTGGGGCCCCCCGCC
GCAAGGGGGACACGGGACACAATTGTTAAACCCTTCCTTTTTTTTTCCCAAACGGGTTAG
AAAAGGAAAAATATTTGGGGTCGGGAAAAAAAA
   
Sequence Name 16-K113258w-SALK_135222C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K113258w-SALK_135222C-R210
TGTTGTCGGACCGATATGTTTTCCTTTGCATGACCGGTCTGGAACTGAATAGTGATGGTT
TTAGCATTTGTGTCCGTTGTCCCATTTGCGACGGGGGACTCAAAGGTTAAGTTTTTAGTA
ACTGCAGTGAGATTTTCCGGCGCCGGTGTACGCAATGTGGTGAAATTAGAATCGAGACTC
GACGGTGAAGAGTTGGATGAATTCGAGACAGTGATATTAGGGAGAACATAGGAAGAAATG
GAAGGAGCGAGTGCGACTGTGATGGGAGAAGGACTAAAGGCTAAGAAAACAGTCAAGGCA
ACGAAAGTTACTACGAAAGCGTAGACGAAAGTTGAAGTTTTTCTTGGTTTCACGGTGGAG
AAAAAGCTTTTGATTTCGACGGTGACGGAGGAGAAGGCGGAGACGCCGTGAATAGGCAAA
TGCTTAACGGAGTCCAACGCCATGGCGAGGGAGTTTTTTGGCGCGTGAGAATCTTTTTCT
CTGACTCTTGGTTTTGCGTTTTTTTTTTTTTTTAAAATTTCAAAAAATTAAAAAAGGGGT
TTTTTTCCCGAAGGGGGAAAAAAAAAAAAATTTTTTTCCCCCAAAAAAAATTTTTTTTTT
TAAAAATTGAAAAAAAAAAAATGTTTTTCTCCGAAAAAGGAACTTTTCTTAAAAAACCCC
CCCAAAATTTGGGGGTTTTTCTTTTTATTTTAGCCCCTTTTTTTTTAAAAACACCTAAAA
AAATCCCCTTTTTTTGTAACGCAAAAAATTTGGAAATGTGAAATTTTTTTTTTGTGGG

Last Updated on 06.12.2018 16:11