Sequences from confirmation amplicon


Confirmation sequences for line SALK_135697c and hit At3g13430 are shown.
Line ID SALK_135697c
Locus At3g13430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4368586
Primers used for PCR 32td - LBb3, show primer details
   
Sequence Name 79-K116811-SALK_135697C-32td
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 32td: 5'-AAATTGAAGCAAACCAGAAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K116811-SALK_135697C-32td
AACACATTGCGGACGTTTGTAATGTCCTGGGGTGGTTCTTCTTTTCACTAATGAGACGGG
CAACCTCCGATTGCTCTTCACCGTCTGG
   
Sequence Name 79-K116810-SALK_135697C-R210
GenBank Accession LN486211 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K116810-SALK_135697C-R210
TGTTATTAGACTGATGCAGAAACATCGAGAAACGATGATAACAATGAAGATATCAGTAAT
GCTTCTATGGCTAGTAATGGATCATCACCAGACTCAAGTTCGAACAATTCATCGGTTAAC
TAAACTAAGGCTCACTATCTGAGACAGGTTTACTTGAACAGAGGAGAACTGATGGGATCG
TTTGAGATCAGTCTTTAGTGTTTAATGTTATTTGGTTTGCACATAGTTTCTCAGATTTGG
TGGAAAATGTATTCTTTGGAATTAATTAACAGTCTTTTTTTTTTTTGACCTTACCTTTTC
CTTCCGGGTT

Last Updated on 06.12.2018 16:11