Sequences from confirmation amplicon


Confirmation sequences for line SALK_135734c and hit At1g56010 are shown.
Line ID SALK_135734c
Locus At1g56010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20947873
Primers used for PCR 43ux - LBb3, show primer details
   
Sequence Name 36-K117668-SALK_135734C-R210
GenBank Accession LN485241 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117668-SALK_135734C-R210
TTTGTTTCTAACAAAGGACAAATTTATACTATTGCGAGACAAAAAAGTTGGCGCTAACAT
TTAATCACATGATCAGTTTCATTATCCTGTAGTGTATAATACAACTAAGCGTGTTTGTGT
GTATGTTTCAAACAAATGATTAGAAATGGCATGCGTGGGAGGGAAGGATTGGTATTTCTA
CAGCCAAAGAGACCGAAAATACGCGACGGGGCTGAGAACTAACCGAGCAACGGCCACCGG
ATATTGGAAAGCCACCGGCAAAGACAGAACCATTCTAAGAAAGGGTAAGCTAGTTGGGAT
GAGGAAGACATTGGTTTTCTATCAAGGTCGAGCTCCTCGAGGCCGTAAAACCGATTGGGT
CATGCACGAATTCCGTCTCCAAGGATCTCATCATCCTCCCAATCATTCTCTGAGCTCTCC
AAAGGTATACACAGTTAAATAATCCAGTTATTGATCGTCAGTTAATCTCTTCTTAGCAAA
TCTGTAACGAGTAATATACGTGTTTATGTGTTGTTGAGGGAATCAGGAAGACTGGGTCTT
GTGTAGGGTATTCCATAAGAATACGGAAGGAGTTATATGTAGAGACAACATGGGAAGCTG
TTTTGATGAGACAGCCTCTGCATCGCTTCCTCCACTGATGGATCCTTACATCAACTTTGA
CCAAGAACCCTCTTCTTATCTCAGTGATGATCATCACTACAT
   
Sequence Name 73-K117669-SALK_135734C-43ux
GenBank Accession LN486089 [GenBank]
Sequencing primer gene specific primer, 43ux: 5'-TGCTCATTGATGATGTAGTGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K117669-SALK_135734C-43ux
AGTTGATGTAAGGATCCATCAGTGGAGGAAGCGATGCAGAGGCTGCTCTTTCAAAACAGC
TTCCCATGTTGTCTCTACATATAACTCCTTCCGTATTCTTATGGAATACCCTACACAAGA
CCCAGTCTTCCTGATTCCCTCAACAACACATAAACACGTATATTACTCGTTACAGATTTG
CTAAGAAGAGATTAACTGACGATCAATAACTGGATTATTTAACTGTGTATACCTTTGGAG
AGCTCAGAGAATGATTGGGAGGATGATGAGATCCTTGGAGACGGAATTCGTGCATGACCC
AATCGGTTTTACGGCCTCGAGGAGCTCGACCTTGATAGAAAACCAATGTCTTCCTCATCC
CAACTAGCTTACCCTTTCTTAGAATGGTTCTGTCTTTGCCGGTGGCTTTCCAATATCCGG
TGGCCGTTGCTCGGTTAGTTCTCAGCCCCGTCGCGTATTTTCGGTCTCTTTGGCTGTAGA
AATACCAATCCTTCCCTCCCACGCATGCCATTTCTAATCATTTGTTTGAAACATACACAC
AAACACGCTTAGTTGTATTATACACTACAGGATAATGAAACTGATCATGTGATTAAATGT
TAGCGCCAACTTTTTTGTCTCGCAATAGTATAAATTTTGTCCTTTTGTTTAGAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
C

Last Updated on 06.12.2018 16:11