Sequences from confirmation amplicon


Confirmation sequences for line SALK_136827c and hit At2g38660 are shown.
Line ID SALK_136827c
Locus At2g38660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16167204
Primers used for PCR 69hw - LBb3, show primer details
   
Sequence Name 31-K112874-SALK_136827C-69hw
GenBank Accession LN485148 [GenBank]
Sequencing primer gene specific primer, 69hw: 5'-CAGAGACTGAACAGAAGACTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112874-SALK_136827C-69hw
ATCAACAAGATATTAGTGAAGTGGGGAAGATGAAAAGGCTGTTGGAAAAGTCCCTGGTCT
TGCTGTTGTTTTGGTTGGTGAACAAAGAGACTCGCAAACTTATGTTCGCAACAAAATTAA
AGCTTGTGAAGAAACTGGCATTAAATCTGTTTTGGCTGAATTACCAGAGGATTGTACTGA
AGGACAGATTATCAGTGTATTGAGAAAATTCAATGAAGACACGTCTATTCACGGAATTCT
TGTGCAACTTCCTTTACCCCAAGTATGTTTCTCATTTTTTTCTTTGGACGTTGTGATGGG
GGATGTTAGTGTTGTTGATGTCAAAGATTAGTTCTATGTTAGGAGGTCAAGTAGTTACGT
TTACTTTGTTTTCATACTTGTATGTAGAAATTCTTGATTCATATATTTTCATCGACCATG
GACTCATGCATAACATTTTCCCCTTTAAGGTTTCCTTTTCGTTGGTAGGTAAAGCATTTA
ATTTACAGGAGAACAAATAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 23-K112873-SALK_136827C-R210
GenBank Accession LN484948 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K112873-SALK_136827C-R210
TTGTTCTCCTGTAAATAAATGCTTTACCTACCAACGAAAAGGAAACACTTTATAAAAAAA
ATGTTATGCATGAGTCCATGGTCGATGAAAATCTATGAATCAAGAATTTCTACATACAAG
TATGAAAACAAAGTAAACGTAACTACTTGACCTCCTAACATAGAACTAATCTTTGACATC
AACAACACTAACATCCCCCATCACAACGTCCAAAGAAAAAAATGAGAAACATACTTGGGG
TAAAGGAAGTTGCACAAGAATTCCGTGAATAGACGTGTCTTCATTGAATTTTCTCAATAC
ACTGATAATCTGTCCTTCAGTACAATCCTCTGGTAATTCAGCCAAAACAGATTTAATGCC
AGTTTCTTCACAAGCTTTAATTTTGTTGCGAACATAAGTTTGCGAGTCTCTTTGTTCACC
AACCAAAACAACAGCAAGACCAGGGACTTTTCCAACAGCCTTTTTCATCTTCCCCACTTC
ACTAATAATCTTTGTTCTGATTTCCTCAGCGATAACATTACCATCTATCACCACAGTCTT
CTGTTCAGTCTC

Last Updated on 06.12.2018 16:11