Sequences from confirmation amplicon


Confirmation sequences for line SALK_137192c and hit At4g26180 are shown.
Line ID SALK_137192c
Locus At4g26180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13260590
Primers used for PCR 18mh - LBb3, show primer details
   
Sequence Name 17-K114334-SALK_137192C-18mh
GenBank Accession LN484751 [GenBank]
Sequencing primer gene specific primer, 18mh: 5'-TCCTGAGAATCAACAATCTGTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114334-SALK_137192C-18mh
TCACAAAACGTATAAGCAATTATGTGTTACATAGAGAGGTCCTAGAGTTTTCAATTGGGA
TTCTGACACTGTTTATATGGAGGAGTGTTAGCTTCAGTTTCCTCTACCAAAAAATGGAAT
GTACAATCTAGTATAATAATATGTGGTTTTAAACACCACAAAGAATCAGATCATATGGTT
GATCTTAGTATTAGGTGAAAACTTTCCTTTTTCGTGTCGTCACAGCTTCTGCCTCAGGTT
CCTCTCGAGGCGGGACTCTNAAGTGCAGCTTCATAATATCATACACTGTGAATCCGATTG
CCACAGACGGTACAACCTAAAGAAACCCGAAAGGAAAAGTGTTTGTAACTTGGTTGTTCA
TTGTCCGAAAACAAAGATAGATCGATGAAACTAGTCTCAAACCTTAAGGTAATTGATGCT
AAGCCCTGAGAAGAGTTGCTTCCAACCTTCTTCTCTTGCAATCTTGAAAAGGGTTTGCAT
TGTTCCTCTTCTTGTTTCTTCCTTAACGGCTGAATATATNGTGGTGTAAACAAATTGACG
CTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTNTGCCCCAGCAGGCGAANATCCTGTTTGATG
   
Sequence Name 07-K114333-SALK_137192C-R210
GenBank Accession LN484466 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114333-SALK_137192C-R210
TATATTCAGCCGTTAAGGAAGAAACAGAAGAGGAACAATGCAAACCCTTTTCAAGATTGC
AAGAGAAGAAGGTTGGAAGCAACTCTTCTCAGGGCTTAGCATCAATTACCTTAAGGTTTG
AGACTAGTTTCATCGATCTATCTTTGTTTTCGGACAATGAACAACCAAGTTACAAACACT
TTTCCTTTCGGGTTTCTTTAGGTTGTACCGTCTGTGGCAATCGGATTCACAGTGTATGAT
ATTATGAAGCTGCACTTAAGAGTCCCGCCTCGAGAGGAACCTGAGGCAGAAGCTGTGACG
ACACGAAAAAGGAAAGTTTTCACCTAATACTAAGATCAACCATATGATCTGATTCTTTGT
GGTGTTTAAAACCACATATTATTATACTAGATTGTACATTCCATTTTTTGGTAGAGGAAA
CTGAAGCTAACACTCCTCCATATAAACAGTGTCAGAATCCCAATTGAAAACTCTAGGACC
TCTCTATGTAAACACATAATTGCTTATACGTTTTTGTTGAATACTTTCCAATGTATCTNA
CCTTACGAAATACTGAGACACAGA

Last Updated on 06.12.2018 16:11