Sequences from confirmation amplicon


Confirmation sequences for line SALK_137266c and hit At4g09900 are shown.
Line ID SALK_137266c
Locus At4g09900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6223563
Primers used for PCR 22gq - LBb3, show primer details
   
Sequence Name 45-K112623-SALK_137266C-22gq
GenBank Accession LN485394 [GenBank]
Sequencing primer gene specific primer, 22gq: 5'-TTAAAGTCCACTTCTTTGGGCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K112623-SALK_137266C-22gq
GGGTACGAGTATCTGCATGAAGAAGAAGATGTTGTTATCAGAAGTGGAGGAGATGGGTCC
AGAAGCAAGAGAGTGAATCGTTCACAGAGGAAGTTGCTTGCTGATGAAGAGAATTTGCAT
CGAAGAGCTTTGTCTATGGCTATTCATCAAGCTCAAGTTTCTCAGAGATTTGATGGATCC
ATGTCTAGACGAATCGGGTCTACTAGCTCTAGAAGAGGAACACTCTCTGACTCATTTTCT
AATAACAAGCAGGTCCAAGCTTTTTGATAGTAATGATAAAGTTTGGATTTTTAAACTTTG
AGGAGAGAATTCAAAAGTATTTATAAAGGTTTTAAATTTGATATTGTTCTTAAAAGGACG
CTTAGACAAATTTACGCAGCAAATCCAAACTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATGGGGCAAAA
   
Sequence Name 21-K112622-SALK_137266C-R210
GenBank Accession LN484883 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112622-SALK_137266C-R210
TTGGATTGCTGCGTAATTTGTCTAAGCGTCCTTTTAGAACAATATCAAATTTAAAACCTT
TATAAATACTTTTGAATTCTCTCCTCAAAGTTTAAAAATCCAAACTTTATCATTACTATC
AAAAAGCTTGGACCTGCTTGTTATTAGAAAATGAGTCAGAGAGTGTTCCTCTTCTAGAGC
TAGTAGACCCGATTCGTCTAGACATGGATCCATCAAATCTCTGAGAAACTTGAGCTTGAT
GAATAGCCATAGACAAAGCTCTTCGATGCAAATTCTCTTCATCAGCAAGCAACTTCCTCT
GTGAACGATTCACTCTCTTGCTTCTGGACCCATCTCCTCCACTTCTGATAACAACATCTT
TCTTCTTCATGCAGATTACTCTGTTACCCATTCTTCACAAAACCCTGAATTTAGCCCAAA
GAAGTGGACTTTA

Last Updated on 06.12.2018 16:11