Sequences from confirmation amplicon


Confirmation sequences for line SALK_137492c and hit At1g20200 are shown.
Line ID SALK_137492c
Locus At1g20200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7004380
Primers used for PCR 40ea - LBb3, show primer details
   
Sequence Name 46-K111105-SALK_137492C-40ea
GenBank Accession LN485413 [GenBank]
Sequencing primer gene specific primer, 40ea: 5'-ACAACCTAAAGCCAGATGCTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K111105-SALK_137492C-40ea
GTGAAGAAGTAAACCCACAAATACCTTAGGAACAAAGGAAGAGAGGCGAGAGTGAGCTTC
GGATCCTGGAACCAAAGCAAAATCCAGGAAGGAAGAGAGCACAGAGCCGGTGAGTTTCTG
CCTAAGCCCTATAGTGAGACGCACAGCACGAGCAATACGACGAACCTCCTTCGTGTAAGA
CCCAGTATCGATGAGTGCTGCAATCTCCTTCAAATCTGGGAAACAAAGAAACAAAGCGTC
AAAACCCAGAAGAAACTTAGCAATTATGAAAACAGTTCGAGAATTAAAGCATTCAGATCG
AGATTTACTCTGCATAGTAGAGGTAGAAGAAGAGATAATCGATTGAGAAGGGGTGTTGTT
ATCTTTCATCTCCACGTCCTGAGTCATCTTTGCACAAATCTCGTTACTCTTCACAAAGAC
AAAAACAAGGTTAGGGTTTTTGCAGAAGTATGCAACTAAGCCGGTGAGATTTCAAGGAAC
CGATTGAAGTTTCAACCAATTTGTTGACTGAGAAAGACGGAGAGATAAAGACAGAGAGCT
TACTAAATCGATAAAACGGAATTCGCCGGGAGAAAGTGACGATGATGGATCAGATTTAAG
GACGAGCTGATGCACTTAATAACACAACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 45-K111120-SALK_137492C-R210
GenBank Accession LN485392 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K111120-SALK_137492C-R210
GTCATTTGTTGTGTTATAAGTGCATCAGCTCGTCCTTAAATCTGATCCATCATCGTCACT
TTCTCCCGGCGAATTCCGTTTTATCGATTTAGTAAGCTCTCTGTCTTTATCTCTCCGTCT
TTCTCAGTCAACAAATTGGTTGAAACTTCAATCGGTTCCTTGAAATCTCACCGGCTTAGT
TGCATACTTCTGCAAAAACCCTAACCTTGTTTTTGTCTTTGTGAAGAGTAACGAGATTTG
TGCAAAGATGACTCAGGACGTGGAGATGAAAGATAACAACACCCCTTCTCAATCGATTAT
CTCTTCTTCTACCTCTACTATGCAGAGTAAATCTCGATCTGAATGCTTTAATTCTCGAAC
TGTTTTCATAATTGCTAAGTTTCTTCTGGGTTTTGACGCTTTGTTTCTTTGTTTCCCAGA
TTTGAAGGAGATTGCAGCACTCATCGATACTGGGTCTTACACGAAGGAGGTTCGTCGTAT
TGCTCGTGCTGTGCGTCTCACTATAGGGCTTAGGCAGAAACTCACCGGCTCTGTGCTCTC
TTCCTTCCTGGATTTTGCTTTGGTTCCAGGATCCGAAGCTCACTCTCGCCTCTCTTCCTT
TGTTCCTAAGGTATTTGTGGGTTTTACTTCTTCACTTTGATGTTTCAGAATTCTCAACTT
GGTTGTTCCTTTTAGCATCTGGTTTTAAGGTTG

Last Updated on 06.12.2018 16:11