Sequences from confirmation amplicon


Confirmation sequences for line SALK_137617c and hit At5g02840 are shown.
Line ID SALK_137617c
Locus At5g02840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:649152
Primers used for PCR 67ns - LBb3, show primer details
   
Sequence Name 74-K114703-SALK_137617C-67ns
GenBank Accession LN486111 [GenBank]
Sequencing primer gene specific primer, 67ns: 5'-GGATATCGTCGTCATTTCTCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K114703-SALK_137617C-67ns
ACGATCTCTATCTTGAATAAAATACCGATAATGACCTCAACCAATCCGGTGGTCGCCGAA
GTAATACCGGCGGAAACTTCTACAGATGCTACAGAGACGACGATTGCAACGACGGAAGCT
GGTGAAGCACCGGAGAAGAAGGTGAGGAAAGCTTACACAATCACCAAGTCTAGAGAGAGT
TGGACTGAAGGAGAACACGACAAGTTTCTGGAAGCTCTTCAATTGTAATTTTACCGCGCT
CTTTTTTTCTTCCACCAGCTATTTGATTGACTGATTCTTTGTGTTTCGAGCTCTAATTTA
TAGCTGAATTTTGCGTAATTTCGCGTAATTTCTACTTAATTTGCTTAATCAGATCGAAGT
TTGGTTATGTGGTAGATCATCTGGAGTTTTGCAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAAT
   
Sequence Name 65-K114702-SALK_137617C-R210
GenBank Accession LN485879 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K114702-SALK_137617C-R210
CATTTGTTTGCAAACTCCAGATGATCTACCACATAACCAAACTTCGATCTGATTAAGCAA
ATTAAGTAGAAATTACGCGAAATTACGCAAAATTCAGCTATAAATTAGAGCTCGAAACAC
AAAGAATCAGTCAATCAAATAGCTGGTGGAAGAAAAAAAGAGCGCGGTAAAATTACAATT
GAAGAGCTTCCAGAAACTTGTCGTGTTCTCCTTCAGTCCAACTCTCTCTAGACTTGGTGA
TTGTGTAAGCTTTCCTCACCTTCTTCTCCGGTGCTTCACCAGCTTCCGTCGTTGCAATCG
TCGTCTCTGTAGCATCTGTAGAAGTTTCCGCCGGTATTACTTCGGCGACCACCGGATTGG
TTGAGGTCATTATCGGTATTTTATTCAAGATAGAGATCGTCTAGAGAGAGAGAGACTGAA
GAGAGAAGGGGTTAGAGAGAGAAATGAC

Last Updated on 06.12.2018 16:11