Sequences from confirmation amplicon


Confirmation sequences for line SALK_137776 and hit At4g33040 are shown.
Line ID SALK_137776
Locus At4g33040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15941154
Primers used for PCR CE51 - R204, show primer details
   
Sequence Name 61-K107135-SALK_137776C-CE51
GenBank Accession LN485756 [GenBank]
Sequencing primer gene specific primer, CE51: 5'-TCGACAAATCGTATTCATATCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K107135-SALK_137776C-CE51
GAATAATTACTCATAAGTCCGATTAAAATCACAACTACTCAGACGACTTATATATATCCA
GATCCTATATATCAGAAGAAAAGAAAAGAAAAACAATTACCAATTCAAGTAAAAGGTTTC
TGAGCTAAATTGTTAGATCGTTAGGTATAAACATCTTGCGTTTTATCAATAAACTTATAT
TTTGTATATGGTTCGAATCTCTTAGTCAAATATTGTGAAAAAAGGAATAAAGAAATTTCA
ATCATTAGATATACAACTAGATCATAGTTTAGTAGTCCTAATCAATCCGATTCATCATTA
ATGAGGCTGATGGATTCATACTTTATCAAAAGAAGCAAATCATATAAAATTTACTAATTA
CACATCTTTAATTATTCATAATTCATTTTCTACATGGGTGTTTAATTATGATCATCTGAT
GATAATCAGAATTTGGCTTTCATGACCAATTGAAGTGAAACCCTAATTTTTGTTTTCTTT
TGATACTGCAAAAGCCTATTGATTTTAACACAAGTAAGAAGACGATGAAAATTACAATCA
TACCCAAAGAGCTCCAACTTGGACAAGCTTAGGAACAAGTTGACCACTTAAGTGAAGAGC
GACAAGGGACTCAAGACCTCCGACGCACTCACGGCCAATGAAAACCGCCGGCGGAGGACC
AACGACGGAGACGCCACCGGAATATTCATCTTGTAGAGCCGTGGGAAGAGAGGAAACCTC
GTGATCATCGAGCTCGATGACGGTGGGGATTACGCCGATCGTTGCTAAGAGTCTCTTCAT
GACGTGGCACATGCAACATGAAGATCTACTGAAGATGATCACAGGATGCTCCGATATCAG
CCGTCGGATCTTGGCTTCCGTTGATTCCTCAGCGATATATTGTGGTGTAAACAAATTGAC
GCTTAGACAACTTAATAACACATTGNCGACGTTTTTATGTACTNGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCT
   
Sequence Name 53-K107134-SALK_137776C-R210
GenBank Accession LN485572 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K107134-SALK_137776C-R210
GTCATTTGTTTCACCACAATATATCGCTGAGGAATCAACGGAAGCCAAGATCCGACGGCT
GATATCGGAGCATCCTGTGATCATCTTCAGTAGATCTTCATGTTGCATGTGCCACGTCAT
GAAGAGACTCTTAGCAACGATCGGCGTAATCCCCACCGTCATCGAGCTCGATGATCACGA
GGTTTCCTCTCTTCCCACGGCTCTACAAGATGAATATTCCGGTGGCGTCTCCGTCGTTGG
TCCTCCGCCGGCGGTTTTCATTGGCCGTGAGTGCGTCGGAGGTCTTGAGTCCCTTGTCGC
TCTTCACTTAAGTGGTCAACTTGTTCCTAAGCTTGTCCAAGTTGGAGCTCTTTGGGTATG
ATTGTAATTTTCATCGTCTTCTTACTTGTGTTAAAATCAATAGGCTTTTGCAGTATCAAA
AGAAAACAAAAATTAGGGTTTCACTTCAATTGGTCATGAAAGCCAAATTCTGATTATCAT
CAGATGATCATAATTAAACACCCATGTAGAAAATGAATTATGAATAATTAAAGATGTGTA
ATTAGTAAATTTTATATGATTTGCTTCTTTTGATAAAGTATGAATCCATCAGCCTCATTA
ATGATGAATCGGATTGATTAGGACTACTAAACTATGATCTAGTTGTATATCTAATGATTG
AAATTTCTTTATTCCTTTTTTCACAATATTTGACTAAGAGATTCGAACCATATACAAAAT
ATAAGTTTATTGATAAAACGCAAGATGTTTATACCTAACGATCTAACAATTTAGCTCAGA
AACCTTTTACTTGAATTGGTAATTGTTTTTCTTTTCTTTTCTTCTGATATATAGGATCTG
GATATATATAATTCGTCTGAGTAGTTGTGATTTTAATCGGACTTATGAGTAATTATTTCT
TTGGTAATTAGAAATCTTTTATAATCTGATGATG
Primers used for PCR 40xo - LBb3, show primer details
   
Sequence Name 29-K119303-SALK_137776C-R210
GenBank Accession LN485120 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K119303-SALK_137776C-R210
GCGTCATTTGTTTACCCACAATATATCCTGAGAACTAAGTGAGGTCTAAGCGAACGACGT
CGTTTGAGAAACGTTGTAAGCCTAATTCTTGCATCATAGCAGGCAATTAGAGAGAGAGAG
AGAAACGTAAAGAGCTTTTTCAAGAAGAAGAGGAGATTTTGTATTTGTATATATAGAAAG
AATCAAAAAGAAGTTTAAAACAAAAGAAGGAATCATAGAGGAAATAAATACAAGGGCCAA
TAAAATAAGAAGAGAATTATTGGGGAAAGAAAAAGAAAAGGACAAACAATGGGGAGGAGA
AGAAGGGAGTGGTGTATGTGCTTGCTTACATCACAAAGTACTGTCTCGTGATGTGTTTCT
TTTGCTTTCGTTTTTTTTTTTGGGGTTTAAAAAATTTTTTTTTTCCGGAGGATTAAAGGG
GGGGGGGTTCCAAAATTCCAAATCCCCCCCCCCAAATTTTTTTGGCCAATGTGGCCTTTT
CCAAAGCCAAAGTTAAAGGGAAAAAAATTTTCCCCATTTGGGACCATAAAGGGGTTTGGG
ACACCTTTTTCCCCCCTTCGGGTGGGGAAAATCCTTTTTGCCAAAAAAAATAAAAAATTT
TTTTTTTTACTTAAAAGAAATTGTTTTGAAACCCAAGGGTCCTTAATTTAAAAAATTCCA
TTTGGGGTCCTAAAAAATACCAACCTTTGGCTTAAGGGGAATTTTTTTTAAAATTTTCCC
ATTCCCCAAAGGTTTTTTTTTTTTTCCAAAAT

Last Updated on 06.12.2018 16:11