Sequences from confirmation amplicon


Confirmation sequences for line SALK_137814c and hit At1g09780 are shown.
Line ID SALK_137814c
Locus At1g09780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3168010
Primers used for PCR 28fr - LBb3, show primer details
   
Sequence Name 26-K115143-SALK_137814C-28fr
GenBank Accession LN485037 [GenBank]
Sequencing primer gene specific primer, 28fr: 5'-CTGTAGTTGATCGAGACGAGAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115143-SALK_137814C-28fr
ACAGATGCAAGTATTCGTCTCGAAAGATTCAGAAACGTATTTAAAACCTTCACCTTCAAA
GATTTTGCCAGAAGCAAGAGCTTGGTCACAAAGCTTAGCACCTTGAGCAAAGATACGACC
AGCACCAAGAGCATTATGACCAACCTCACTGTTTCCCATATCATCTTCACTAGGAAGTCC
TACAGCAGTACCGTGAGCTTTGATCAAAGTCCAAGTATCAGGAGCTCCCTATAGAGAAAC
AAGAGTAAGTTCAATGAATCCAAAAAGCATCAAAAATCAAAGATTAAGGAGGTAACATAC
ATGTTTAAGAGAATCCATGGCTGGAGTTGGAGCGTTGTGGATACAGTTGTACTGATCAGG
GGCAGATTCACCCCAACCATCTNAGACGATTACGGCGATGGTCTTACCCTTGGGGAGCTT
GGGGTGGTCATCGAGCTTCCAAGCGGAGGAGGTAGCCATAGCTGAGATTTTTTGAGATGC
AATCGGAGAATCTCAAAGCAGATCTGAGAGAAAAGTGAAGGTTGGTGTGAAGGAAGAGGC
GGGTTTTAAAAAGAGGCGTTTTTGCAATATGGGCCCGTCAATTGGGCCACTTGTCTCTCA
CGTAAATATAAAATTCTTATTGGCTCCGAATATTCTTTTTCCTTATTTTTCTTATGATAT
ATTGTGGTGTAAACAAATTGACGCTGAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTGCCCCAGCAGGCGAAAA
TCCNGTTTGATGGTGGTTCCG
   
Sequence Name 10-K115142-SALK_137814C-R210
GenBank Accession LN484559 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115142-SALK_137814C-R210
TTTGTTTACCCACAATATATCATAAGAAAAATAAGGAAAAAGAATATTCGGAGCCAATAA
GAATTTTATATTTACGTGAGAGACAAGTGGCCCAATTGACGGGCCCATATTGCAAAAACG
CCTCTTTTTAAAACCCGCCTCTTCCTTCACACCAACCTTCACTTTTCTCTCAGATCTGCT
TTGAGATTCTCCGATTGCATCTCAAAAAATCTCAGCTATGGCTACCTCCTCCGCTTGGAA
GCTCGATGACCACCCCAAGCTCCCCAAGGGTAAGACCATCGCCGTAATCGTCTTAGATGG
TTGGGGTGAATCTGCCCCTGATCAGTACAACTGTATCCACAACGCTCCAACTCCAGCCAT
GGATTCTCTTAAACATGTATGTTACCTCCTTAATCTTTGATTTTTGATGCTTTTTGGATT
CATTGAACTTACTCTTGTTTCTCTATAGGGAGCTCCTGATACTTGGACTTTGATCAAAGC
TCACGGTACTGCTGTAGGACTTCCTAGTGAAGATGATATGGGAAACAGTGAGGTTGGTCA
TAATGCTCTTGGTGCTGGTCGTATCTTTGCTCAAGGTGCTAAGCTTTGTGACCAAGCTCT
TGCTTCTGGCAAAATCTTTGAAGGTGAAGGTTTTAAATACGTTTCTGAATCTTTCGAGAC
GAATACTTTGCATCTTGTTGGACTTCTCAGTGACGGTGGAGTTCACTCTCGTCTCGATCA
ACTACA

Last Updated on 06.12.2018 16:11