Sequences from confirmation amplicon


Confirmation sequences for line SALK_138624 and hit At1g17950 are shown.
Line ID SALK_138624
Locus At1g17950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6179042
Primers used for PCR 02bz - LBb3, show primer details
   
Sequence Name 22-K108287-SALK_138624C-02bz
GenBank Accession LN484906 [GenBank]
Sequencing primer gene specific primer, 02bz: 5'-CAAGTCCTCAAAGAGTTCTTGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K108287-SALK_138624C-02bz
GAAGGTATTAGATAATATGGCTTGAATTTCTGTGTTTATGTAGCTAATATAGCTGCACAA
AGATAAAACATGTGTAGCCATACGTATATTGTATTTTGTGTGAATACATATAGGCATATA
TAAATTACGAACTCATAGAAAACATGTACATTCGTCATATTAATGTGCAAGTAAATGAGT
AATGGTGCATTAATTCGTATACAACTTGATTTAAAAAAAAAAACGATTTTTGTATAGATT
ATATGGGTTAAAATTCATTGTTTTCTGTAAACTCTAAATTAATTTTTGATCAATTTTTTT
TGTAAAGATAGTATGAAACGATCATGATTTTCATAGTTACATGCATGCATATGGATGTAC
ACTTGCATGGGGGTACAATTACATACTTGTGTAACTTCCAAAACTAACTTTTGGACATGG
ATTAATTTGTCAGGAGCAATAGACCAAACTAAACGACCGAGGGAGTTCTACAATTTTCTT
CAAGTAAACACGGATTCGAAAATACCCAAATTGATAGATAATTCAAGAAAAGACGAAGAA
GAAGATGTCGATCAAAACAACCGAATTCCTAACGAGAATTGTGTTCCATTTTTCGACTTT
TTGTCTGTTGGAAACCCTATAACACATTGACCCTTAAACAACTTAATAACACTTTGCGGA
CGTTTTTAATGTACTGGGGGGGTTTTTCTTTT
   
Sequence Name 06-K108286-SALK_138624C-R210
GenBank Accession LN484421 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K108286-SALK_138624C-R210
TGTGTTAAGAGTTCCACAGACAAAAGTCGAAAAATGGAACACAATTCTCCTTAGGAATTC
GGTTGTTTTGATCGACATCTTCTTCTTCGTCTTTTCTTGAATTATCTATCAATTCGTGTA
TCTTCGAATCCGTGTTTACTTGAAGAAAATTGTAGAACTCCATCGGTCGTTTAGTTTGGT
CTATTGCTCCTGACAAATTAATCCATGTCCAAAAGTTAGTTTTGGAAGTTACACAAGTAT
GTAATCGTACCCACATGCAAGTGTACATCCATATGCATGCATGTAACTATGAAAATCATG
ATCGTTTCATACTATCTTTACAAAAAAAATTGATCAAAAATTAATTTAGAGTTTACAGAA
AACAATGAATTTTAACCCATATAATCTATACAAAAATCGTTTTTTTTTTTAAATCAAGTT
GTATACAAATTAATGCACCATTACTCATTTACTTGCACATTAATATGACGAATGTACATG
TTTTCTATGAGTTCGTAATTTATATATGCCTATATGTATTCCCACAAAATACAATATACG
TATGGCTACACATGTTTTATCTTTGTGCAGCTATATTAGCTACATAAACACAGAAATTCA
AGCCATATTATCTAATTACCTTCTTGTATTGGAGTAGTACTATTTCTGAACCCGATCTTT
CCGGTCAAAAACTCTTTAAGAACTTGACGGGAAGGATGGGGTTCGGAAATGGTACTACTC
CAATACAGGAGGGTAATTAGATAATAGGGCTTGAATTTCGGTGTTTATGTGGCTAATATG
GCGGCACAAGGATAAAAC

Last Updated on 06.12.2018 16:11