Sequences from confirmation amplicon


Confirmation sequences for line SALK_139161 and hit At5g45770 are shown.
Line ID SALK_139161
Locus At5g45770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18564859
Primers used for PCR 46wj - LBb3, show primer details
   
Sequence Name 46-K118859-SALK_139161C-46wj
GenBank Accession LN485427 [GenBank]
Sequencing primer gene specific primer, 46wj: 5'-ATCTCACTGGCCTTATCCCTAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118859-SALK_139161C-46wj
ATTTATCAACACTCACTCAAAGGCTCAATACGCATTTCCATTACTCGTCTCAAAAACCTC
AAGTCCTTAAACTTATCTCATAACTCTCTCTCTGGCCAAATACCAAACAAAATCAAAAGC
TTAACTTTTCTAAAAAACTTATCTTTAGCTTCAAACAAACTATCAGGAACAATCCCAAAT
TCACTCTCATCTATCTCTGAACTTACACATTTGGATCTAAGTATGAATCAACTAAACGGA
ACCGTTCCAAGTTTCTTCTCCGAGATGAAGAACCTCAAACACTTGAATCTCGCTGATAAC
TCCTTCCATGGAGTTTTACCATTCAACGAAAGCTTCATCAAAAATCTCAACTTTTTCGAA
ATTGGAAGGAACAGTGAGCTCTGTTATAACAAAACTGTTCTCTCCTCGAACTTGAAATTG
GAGGGTCTTGCTCCGTGTGATAAATACGGTTTTCCTTTATGGTCTCCGTCGCAAAAGGAG
GAATCTTTGTCAGGAGAAAACGATTACGACGTTGAAGGAGGCAATGAGGAGAAGACGGAG
AATCTGAAGACGAAGGAAGAAGAAGAAGAAGAACACAAAGGTTCCAACAAGACTCTGTTT
GGTCTCGGCATTGGTCTTTTCTCGCTTGTGTTCTTAATCCTCTTCCTCTTTTATCTCGCT
AAAAGGTGTCGTTTGATTTGAGAAGTCTGGGACGTTGACGCTTCTAAATGTTGCCAGGAT
ATATTGTGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCNGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
   
Sequence Name 22-K118858-SALK_139161C-R210
GenBank Accession LN484932 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118858-SALK_139161C-R210
GTCATTTGTTTACCACAATATATCCTGGCAACATTTAGAAGCGTCAACGTCCCAGACTTC
TCAAATCAAACGACACCTTTTAGCGAGATAAAAGAGGAAGAGGATTAAGAACACAAGCGA
GAAAAGACCAATGCCGAGACCAAACAGAGTCTTGTTGGAACCTTTGTGTTCTTCTTCTTC
TTCTTCCTTCGTCTTCAGATTCTCCGTCTTCTCCTCATTGCCTCCTTCAACGTCGTAATC
GTTTTCTCCTGACAAAGATTCCTCCTTTTGCGACGGAGACCATAAAGGAAAACCGTATTT
ATCACACGGAGCAAGACCCTCCAATTTCAAGTTCGAGGAGAGAACAGTTTTGTTATAACA
GAGCTCACTGTTCCTTCCAATTTCGAAAAAGTTGAGATTTTTGATGAAGCTTTCGTTGAA
TGGTAAAACTCCATGGAAGGAGTTATCAGCGAGATTCAAGTGTTTGAGGTTCTTCATCTC
GGAGAAGAAACTTGGAACGGTTCCGTTTAGTTGATTCATACTTAGATCCAAATGTGTAAG
TTCAGAGATAGATGAGAGTGAATTTGGGATTGTTCCTGATAGTTTGTTTGAAGCTAAAGA
TAAGTTTTTTAGAAAAGTTAAGCTTTTGATTTTGTTTGGTATTTGGCCAGAGAGAGAGTT
ATGAGATAAGTTTAAGGACTTGAGGTTTTTGAGACGAGTAATGGAAATGCGTATTGAGCC
TTTGAGTGAGTTGTTTGATAAATCTATGTATCTGAGATTTGAGTGGAAAGATTTAGGGAT

Last Updated on 06.12.2018 16:11