Sequences from confirmation amplicon


Confirmation sequences for line SALK_139541c and hit At3g46600 are shown.
Line ID SALK_139541c
Locus At3g46600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17159454
Primers used for PCR AD09 - R204, show primer details
   
Sequence Name 73-K107371-SALK_139541C-AD09
GenBank Accession LN486072 [GenBank]
Sequencing primer gene specific primer, AD09: 5'-GAGCTTTTGAGAAACTGAAGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K107371-SALK_139541C-AD09
TGCGACTCAAGACTTGGTTATCACTTTGCTGAGGCGCTTGAAGCTCGTATTACAGGAACC
ATGACTACACCAATATCTGCTACTTATAGCAGAACATCAATGGTGGACATTTTGAAGGCG
TACAAGGGATTTGTTCAGGCTTGCCCCACCTTAATAATGTGTTATTTCACTGCAAACAGA
ACAATCAATGAGCTTGCTTCCAAAGCAACCACACTTCACATCATTGATTTTGGGATTCTC
TATGGATTTCAATGGCCTTGTCTGATACAAGCTTTGTCAAAACGTGACATCGGACCACCA
CTGCTCCGTGTGACTGGTATCGAGCTTCCTCAGTCAGGTTTCCGTCCATCAGAGCGGGTA
GAAGAGACCGGGCGAAGACTAAAGAGGTTCTGTGACAAGTTCAATGTCCCGTTTGAGTAC
AGTTTCATAGCCAAGAATTGGGAGAACATAACTCTTGATGATCTGGTGATCAATAGTGGC
GAGACAACAGTTGTCAACTGCATCCTCCGGCTACAATATACACCTGATGAAACCGTGTCC
CTCAACTCACCGAGAGACACGGCTCTGAAACTATTCAGAGACATCAACCCTGACCTCTTT
GTGTTTGCAGAGATTAACGGGACTTACAATTCACCCTTCTTCCTAACAAGGTTCAGAGAA
GCTCTCTTCCATTGCGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTNGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
   
Sequence Name 89-K107372-SALK_139541C-R210
GenBank Accession LN486324 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K107372-SALK_139541C-R210
GCGTCATTTGTTTCCCGCAATGGAAGAGAGCTTCTCTGAACCTTGTTAGGAAGCCGTTTA
ATTGTAAGTCCCGTTAATCTCTGCAAACACAAAGAGGTCAGGGTTGATGTCTCTGAATAG
TTTCAGAGCCGTGTCTCTCGGTGAGTTGAGGGACACGGTTTCATCAGGTGTATATTGTAG
CCGGAGGATGCAGTTGACAACTGTTGTCTCGCCACTATTGATCACCAGATCATCAAGAGT
TATGTTCTCCCAATTCTTGGCTATGAAACTGTACTCAAACGGGACATTGAACTTGTCACA
GAACCTCTTTAGTCTTCGCCCGGTCTCTTCTACCCGCTCTGATGGACGGAAACCTGACTG
AGGAAGCTCGATACCAGTCACACGGAGCAGTGGTGGTCCGATGTCACGTTTTGACAAAGC
TTGTATCAGACAAGGCCATTGAAATCCATAGAGAATCCCAAAATCAATGATGTGAAGTGT
GGTTGCTTTGGAAGCAAGCTCATTGATTGTTCTGTTTGCAGTGAAATAACACATTATTAA
GGTGGGGCAAGCCTGAACAAATCCCTTGTACGCCTTCAAAATGTCCACCATTGATGTTCT
GCTAGAAGTAGCAGATATTGGTGTAGTCATGGTTCCTGTAATACGAGCTTCAAGCGCCTC
AGCAAAGTGATAACCAAGTCTTTGAGTCGCATCACCGTGGCGAGAAGAGTGTTCTCTTAT
CTCCTTCAGTT

Last Updated on 06.12.2018 16:11