Sequences from confirmation amplicon


Confirmation sequences for line SALK_139620 and hit At1g56690 are shown.
Line ID SALK_139620
Locus At1g56690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21255798
Primers used for PCR 27de - LBb3, show primer details
   
Sequence Name 10-K109680-SALK_139620C-27de
GenBank Accession LN484545 [GenBank]
Sequencing primer gene specific primer, 27de: 5'-GGTTTTCCTCAAAGGACATTATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K109680-SALK_139620C-27de
TCTAGGAGAGGAAGCTCTTAAGATTTTTCACGAGATGCCATCATCTGGTACCATGCCAAA
CAAAGTGACCTTAATTGCAATTCTGACAGCATGTAGCTATGCTGGAAAACTCGAGGAAGG
GCTTGAGATATTTGAATCAATGGAATCAAAGTTTTGCGTGACTCCAACGGTTGAGCATTA
TTCTTGCACGGTGGATATGCTTGGGCGTGCAGGACAGGTAGATAAGGCAATGGAGTTGAT
CGAAAGTATGACTATTAAGCCAGATGCTACAGTTTGGGGTGCTCTGTTAGGAGCGTGTAA
AACTCACTCACGATTGGATTTAGCTGAAGTTGCTGCAAAGAAACTTTTCGAGAATGAACC
AGATAACGCTGGTACATACGTTTTGCTATCGAGCATAAATGCATCTCGATCCAAGTGGGG
GGATGTTGCAGTAGTAAGAAAAAACATGAGGACAAACAATGTAAGTAAATTCCCTGGTTG
TAGTTGGATTGAGGTAGGGAAGAAAGTGCACATGTTCACTCGCGGAGGTATCAAAAACCA
TCCCGAGCAAGCGATGATACTAATGATGTTGGAGAAAACAGATGGATTATTAAGAGAAGC
TGGTTATAGTCCTGATTGCAGCCATGTCTTACACGATGTAGACGAGGAAGAGAAAGTTGA
TAGTTTAAGCCGTCACAGTGAAAGACTCGCGGTAGCTTATGGACTCTTAAAGCTACCCGA
AGGAGTACCTATTCGAGTCATG
   
Sequence Name 26-K109679-SALK_139620C-R210
GenBank Accession LN485027 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K109679-SALK_139620C-R210
CAGGTTCTTCATGACTCGAATAGGTACTCCTTCGGGTAGCTTTAAGAGTCCATAAGCTAC
CGCGAGTCTTTCACTGTGACGGCTTAAACTATCAACTTTCTCTTCCTCGTCTACATCGTG
TAAGACATGGCTGCAATCAGGACTATAACCAGCTTCTCTTAATAATCCATCTGTTTTCTC
CAACATCATTAGTATCATCGCTTGCTCGGGATGGTTTTTGATACCTCCGCGAGTGAACAT
GTGCACTTTCTTCCCTACCTCAATCCAACTACAACCAGGGAATTTACTTACATTGTTTGT
CCTCATGTTTTTTCTTACTACTGCAACATCCCCCCACTTGGATCGAGATGCATTTATGCT
CGATAGCAAAACGTATGTACCAGCGTTATCTGGTTCATTCTCGAAAAGTTTCTTTGCAGC
AACTTCAGCTAAATCCAATCGTGAGTGAGTTTTACACGCTCCTAACAGAGCACCCCAAAC
TGTAGCATCTGGCTTAATAGTCATACTTTCGATCAACTCCATTGCCTTATCTACCTGTCC
TGCACGCCCAAGCATATCCACCGTGCAAGAATAATGCTCAACCGTTGGAGTCACGCAAAA
CTTTGATTCCATTGATTCAAATATCTCAAGCCCTTCCTCGAGTTTTTCAGCATAGCTACA
TGCTGTCAGAATTGCAATTAAGGTCACTTTGTTTGGCATGGTACCAGATGATGGCATCTC
GTGAAAAATCTTAAGAGCTTCCTCTCCTAAACCATGCGACGCAA

Last Updated on 06.12.2018 16:11