Sequences from confirmation amplicon


Confirmation sequences for line SALK_139663c and hit At4g14070 are shown.
Line ID SALK_139663c
Locus At4g14070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8114639
Primers used for PCR 38gz - LBb3, show primer details
   
Sequence Name 42-K112623-SALK_139663C-38gz
GenBank Accession LN485332 [GenBank]
Sequencing primer gene specific primer, 38gz: 5'-TCAACATGAATGGGTAAACTACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K112623-SALK_139663C-38gz
ACAAAATATATCAGTGTTAACTATTAATATATATCTAACAAAACAAAATAGAAAATAGAA
TTCGCAAACTACCTTCGATATCCCAATAGACGAATGAATTTTCTTGTAGATAAGCTTTTT
GGCCAACATATGTAATGGCCACAACAAGGCAGCAATTACTCTCGCCCACAACCAATCCAC
AAAAGCAACAATATACATTGGAGGCTTTTGCTCTTTTGTCAGACACATACCCTGTAAACC
AGACCAAAAAATCAAAAGTTCAGGAACCACAAAAAGGGTAATCAGAAAAACTCTTAAAAC
AAACTATAACGTAAGAACAAGTGTTAAAAAGGTATCATGAACCTCATATATCCTTTTCAT
CTCCATATATGCCATACTGACTTTGATCAATGTAAGTGCTAGAAATTTACGGCCAGCAGA
ACTTGCAGAAATTTGCTTTTGAATCCCACTACAGAGCTCAAAGAGGAAACAAAGAAACGA
ATGACAAAACTTGTTAAGAACCAACGCATAGAATCCATCATTATATATAATATGAATATT
GTGCAAACTTTTAACTTGAAAAATGACAAACCTGTAAAGTGTCTCATATACTAGAGGAAC
GGACACAATATAGTTCGGTTGGTACCGCTTTAGATCATCCTATACCAAAAGAAAACAAAA
ATAAAAAGGAATTAGAGCCAAAAGAAGCAACGCTNAATGAATTACTTGATACATTCTGCA
AATTTCACATTTTCCCACTTTCCAGTGTTTCATAACTACTATGGCACAATGACAAATTGA
CGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAATCCTGTTTGATGG
   
Sequence Name 18-K112622-SALK_139663C-R210
GenBank Accession LN484780 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K112622-SALK_139663C-R210
GTCATTTGTCTTGTGCCTAGTAGTTATGAAACACTGGAAAGTGGGAAAATGTGAAATTTG
CAGAATGTATCAAGTAATTCATTTAGCGTTGCTTCTTTTGGCTCTAATTCCTTTTTATTT
TTGTTTTCTTTTGGTATAGGATGATCTAAAGCGGTACCAACCGAACTATATTGTGTCCGT
TCCTCTAGTATATGAGACACTTTACAGGTTTGTCATTTTTCAAGTTAAAAGTTTGCACAA
TATTCATATTATATATAATGATGGATTCTATGCGTTGGTTCTTAACAAGTTTTGTCATTC
GTTTCTTTGTTTCCTCTTTGAGCTCTGTAGTGGGATTCAAAAGCAAATTTCTGCAAGTTC
TGCTGGCCGTAAATTTCTAGCACTTACATTGATCAAAGTCAGTATGGCATATATGGAGAT
GAAAAGGATATATGAGGTTCATGATACCTTTTTAACACTTGTTCTTACGTTATAGTTTGT
TTTAAGAGTTTTTCTGATTACCCTTTTTGTGGTTCCTGAACTTTTGATTTTTTGGTCTGG
TTTACAGGGTATGTGTCTGACAAAAGAGCAAAAGCCTCCAATGTATATTGTTGCTTTTGT
GGATTGGTTGTGGGCGAGAGTAATTGCTGCCTTGTTGTGGCCATTACATATGTTGGCCAA
AAAGCTTATCTACAAGAAAATTCATTCGTCTATTGGGATATCGAAGGTAGTTTGCGAATT
CTATTTTCTATTTTGTTTTGTTAGATATATATTAATAGTTAACACTGATATGATTTTGGT
TTCCACAGGCTGGTATTAGCGGAGGTGGTAGTTTACCCATTCATGTTGA

Last Updated on 06.12.2018 16:11