Sequences from confirmation amplicon


Confirmation sequences for line SALK_139887c and hit At5g06340 are shown.
Line ID SALK_139887c
Locus At5g06340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1937052
Primers used for PCR 14gj - LBb3, show primer details
   
Sequence Name 05-K112347-SALK_139887C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112347-SALK_139887C-R210
TTATTTAGATCCTACAAGTGCATTCGCCGGGGAGACTCTTGAC
   
Sequence Name 14-K112348-SALK_139887C-14gj
GenBank Accession LN484668 [GenBank]
Sequencing primer gene specific primer, 14gj: 5'-CAGTTTGGTGATACACAGAGCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K112348-SALK_139887C-14gj
GATGAAGGATCTTATTGAAATATAATTTACTATGAGAGAGAGATGTCAAAAGCGTGTCTC
CGCAATGAATTCTGCCGAAGTAACCCCTGTTTCTTCTCTCAGTTCTCTAAAGGCAGCGTT
CCTCAAATCTTCTCCCTCATCAGCACCACCCTGTAAAAGCAGATTTAATAAAAACCTATG
GCCAAAATTGATACCCTAAAGGATCATAAACTGGTGAATCTACAGCCTAAGCTACTTCTC
AGGATTGTCAATGCATTTGGTTTTTACTTTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATGNGCAAAA

Last Updated on 06.12.2018 16:11