Sequences from confirmation amplicon


Confirmation sequences for line SALK_140255c and hit At3g54800 are shown.
Line ID SALK_140255c
Locus At3g54800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20288039
Primers used for PCR 05et - LBb3, show primer details
   
Sequence Name 21-K111285-SALK_140255C-05et
GenBank Accession LN484881 [GenBank]
Sequencing primer gene specific primer, 05et: 5'-GAGCTTTTGAATTTCAGGTTTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K111285-SALK_140255C-05et
AGATGGGATTCCGCGGAGGGTATGCTTATTTGGTCTATTATTGAAATATATGTGAATCAT
AATTGGTTTTTAACTTATTTTCTTTTGCTGAAACCTCTTGCAGCATTGGGATGATCACCC
AGCAATCATGGCAGTTGGGGTCATTGATGGTACTTCAGAGGATATTTTCAACACGTTAAT
GTCTCTCGGTCCCTTACGATCAGAGTAAGCTCTATGACTTTTGATAACTTGTCCTATTAG
GTCGAGTCTCACTTACTTTGTTTAAAAGTTAGGCAATATAGGTCTACATATTATGACTTA
TATTATTAAGAGATTGAAAAATTGCTGGATTTCTTGCAGATGGGACTTCTGTTTCTACAA
AGGAAACGTGGTGGAGCATCTTGATGGACACACAGATATAATCCATCTTCAGTTATACAG
TGATTGGTTGCCATGGGGCATGAATCGAAGAGACTTATTGCTGCGACGCTACTGGAGAAG
GGAAGATGATGGAACATATGGTATGAATCATTCTTGATAATGTTCTTGATTTGTTGAGTT
TTCGGGTTTATAACCTTCTCAACCTTAAAATGTCACAGTCATACTTTGACAAATTGACGC
TTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTC
ACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAT
CGGGCAAA
   
Sequence Name 10-K111284-SALK_140255C-R210
GenBank Accession LN484548 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K111284-SALK_140255C-R210
TTTGTCAAGTATGACTGTGACATTTTAAGGTTGAGAAGGTTATAAACCCGAAAACTCAAC
AAATCAAGAACATTATCAAGAATGATTCATACCATATGTTCCATCATCTTCCCTTCTCCA
GTAGCGTCGCAGCAATAAGTCTCTTCGATTCATGCCCCATGGCAACCAATCACTGTATAA
CTGAAGATGGATTATATCTGTGTGTCCATCAAGATGCTCCACCACGTTTCCTTTGTAGAA
ACAGAAGTCCCATCTGCAAGAAATCCAGCAATTTTTCAATCTCTTAATAATATAAGTCAT
AATATGTAGACCTATATTGCCTAACTTTTAAACAAAGTAAGTGAGACTCGACCTAATAGG
ACAAGTTATCAAAAGTCATAGAGCTTACTCTGATCGTAAGGGACCGAGAGACATTAACGT
GTTGAAAATATCCTCTGAAGTACCATCAATGACCCCAACTGCCATGATTGCTGGGTGATC
ATCCCAATGCTGCAAGAGGTTTCAGCAAAAGAAAATAAGTTAAAAACCAATTATGATTCA
CATATATNTCAATAATATGACCAAATAAGCATTACCCTTCCGCGGGAATCCCAATCTTTA
GCTTCTTTGAAAAGGCGTAAACCTGAAATTCAAAAGCT

Last Updated on 06.12.2018 16:11