Sequences from confirmation amplicon


Confirmation sequences for line SALK_140393c and hit At2g43010 are shown.
Line ID SALK_140393c
Locus At2g43010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17888200
Primers used for PCR 21ay - LBb3, show primer details
   
Sequence Name 41-K107479-SALK_140393C-21ay
GenBank Accession LN485306 [GenBank]
Sequencing primer gene specific primer, 21ay: 5'-AAACATTTTACCAAAATCGTCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K107479-SALK_140393C-21ay
CCTTCCTGAGTCGCGGCCTGATGTGTGGGAACCCCCGATGTATCTAGCAAACCGGTCGGA
GATGATCTGGTTTTGTACCGGGTTTTGGCAAACTAAACCGGGATTGTTCTGAATTGCAGA
CTGATCCATAACCGGAAATCGAGGTAACTGTACCGGGCTCTGTATCTGACGTATGAACTG
CTGAGGTTGAACTCCGGGGAACATCATCGGAGCCGAAGCCGCCGCCGCCGCCATTCCACT
CCCCATCCACATCACTTGAAGCTGTAACTGAAGTGATTTCAAATAATCTATGGCTTCGTC
TAAAATCGAAGCTTTATCAGTCTGCAAAAACACACCAATGAATGAATCAATCTTCATTTC
TTAATCTGATTCACTTTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTT
GATGGTGGTTCCGAAATCGGGCAAAA
   
Sequence Name 57-K107480-SALK_140393C-LBb3
GenBank Accession LN485657 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K107480-SALK_140393C-LBb3
GGCAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCA
GCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGCA
ATGTGTTATTAAGTTGTCTAAAAGTGAATCAGATTAAGAAATGAAGATTGATTCATTCAT
TGGTGTGTTTTTGCAGACTGATAAAGCTTCGATTTTAGACGAAGCCATAGATTATTTGAA
ATCACTTCAGTTACAGCTTCAAGTGATGTGGATGGGGAGTGGAATGGCGGCGGCGGCGGC
TTCGGCTCCGATGATGTTCCCCGGAGTTCAACCTCAGCAGTTCATACGTCAGATACAGAG
CCCGGTACAGTTACCTCGATTTCCGGTTATGGATCAGTCTGCAATTCAGAACAATCCCGG
TTTAGTTTGCCAAAACCCGGTACAAAACCAGATCATCTCCGACCGGTTTGCTAGATACAT
CGGTGGGTTCCCACACATGCAGGCCGCGACTCAGGTAAGGCCAATACGAAGCCGTGTTTA
TTAAACGACGATATTGGAAAAATGTT

Last Updated on 06.12.2018 16:11