Sequences from confirmation amplicon


Confirmation sequences for line SALK_140623c and hit At2g24420 are shown.
Line ID SALK_140623c
Locus At2g24420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10380065
Primers used for PCR 06et - LBb3, show primer details
   
Sequence Name 51-K111283-SALK_140623C-06et
GenBank Accession LN485533 [GenBank]
Sequencing primer gene specific primer, 06et: 5'-TCTGAATGTCAGATTGATGTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K111283-SALK_140623C-06et
GGATGGGTCCAATCGATCAGATAGTATCTTCCATATAGGTCTCAAGAGTTTGAGAAGATG
TCTTTCACCGTTTCATGCGGAAGTTGACTCCTTACTTTAAGCAATTGAATGCATGATCGT
AATTAGTGATATATTTGGAGTTTTCGCTTCCGGTTACTCTGATATGATATCTTTCCTCGA
CAACTATAACGAATGACCAATATTTGTAATAGAGATAGTCTATTTTCGATCTCTCATTTG
TTTCTTTCTTTTTTTAACATTACATTTTTTCATAGAATTCTAATACTCACAGATTGTTTA
ATGATTTTTTCTTACAAAAAGTATCATTCAGATAATTTAATAAAAATGGTATCGCAGTGC
CTTTATTTACCTTTAGGAGTAAGTTTTCTTTCTTCCGATATCCTAAATTGTTCGACACGT
GTCAATCACGAAACCACAACCAAAAAACCTTGTCGTCTTCTCCAATCATAAAAAAAAAAA
AAAAACGGGGCCCCATTTTGATCAAAAAAAAAAATTTCATAATTTCGAAAAAAAAAACAA
AAAATTTTTTTGTGGGAAAATCCCCGGGAAAATCATTTTTTTTTTTTTTTCCCACGAAAT
TTTAAAAATGGGGGGCCGCAAAATTAAAAAACACTTTGGGAACTTTTTAAAGGAACGGGG
GGGGTTTTTTTTTTCCCCAGGAAAAGGGGAAACAGTTAATTGCCTTTCCCCCCCGGCCCT
TAAAAAATTTGCACCAGGGGGCCCACCCTGGTTTGCCCCACCGGGAAAAAATCCGGTTGG
AGGGGGGT
   
Sequence Name 35-K111282-SALK_140623C-R210
GenBank Accession LN485219 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K111282-SALK_140623C-R210
ACTTCCGTGGAACAAATAAGAAAGATGATCTCGCCGGAGATTTGCCACAAGAAGATTTTT
CGTTCTCTTCTCCGAATTTATGAATTTGTTTTGTTTGATCAAATTGGGACACTGTTTTTT
TTTTTTTTTTAGGATGGGAAAAAACAACAGGGTTTTTGGGTGGGGGTTCCGGGATGGACC
CGGGCCAAAAATTTTGGGATTTCGAAAAAAAAAAAATTTCCCCCAAAGGGAAAAAAAGGG
CCCGGCAACCCCTTTTTTATAAAATTACCGAAAGGAACCTTTTGGAAAAAAAAAACCTTA
AAACACCCGGGGAGTTTAAAATTTCTAGAAAAAATGGTAGGGTTAAAAAAAAAAAAAACC
AATGGAGAGACCGAAAATAACCTTTCCCTTTAAAAAAAAGGGGCCATCCTTTAAAGTGGC
CGGGAAAAAATTTCTTTTCAAAGTACCCGGAGGCAAAACCCCCAAATTTTCCCCTAATTC
CAACCGGGCTTTCATTGGCTAAAAGAAGGGAGCCACCTCCCCCAGAAAAGGGGAAAAAAC
TTCTTCCCAACCTTTGGAACCCTTTGGGGAAAATCCTTTTTGGATCGATAAGGCCCCCCC
CGGGTCGGCTAAAATAACCCCCCAGGGGCCGGGAAACTTC

Last Updated on 06.12.2018 16:11