Sequences from confirmation amplicon


Confirmation sequences for line SALK_141699c and hit At1g21190 are shown.
Line ID SALK_141699c
Locus At1g21190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7420441
Primers used for PCR 42cv - LBb3, show primer details
   
Sequence Name 61-K109345-SALK_141699C-42cv
GenBank Accession LN485759 [GenBank]
Sequencing primer gene specific primer, 42cv: 5'-GATTCGGTTGAGTATCGAAGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K109345-SALK_141699C-42cv
ACTCCGCGGCAGCTTCCGTACGTTCTTACCACTCGGATTCCAGAAACCAGTAACCAGATG
TGATCTTTTTCGATTTTGAGCTCCTTTTTGAATTCCTTATTCGTTGAATTTAACACTAGT
TTTTATTCTTTTCACAAATATCAAAATTTGGACCCATTTTGTTGAGTTTCAGTTTCGAAA
GCCTCAGCGAGAGCTAATTGGCGTAGAAAACCTCATAATCTTGTTAAATTCTAAGACTCT
CACTTTTGTTGTTGTTTTTGTGGTGTAGGCTTTTGATCAGCATTTGAATATGATTCTGGG
TGATGTTGAAGAGGTGTAATTCTCTTAGCTCAATAACACATTACGGACGATGAAGAACCT
AAGAGAAAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
ATCGGGCAAAA
   
Sequence Name 53-K109344-SALK_141699C-R210
GenBank Accession LN485576 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K109344-SALK_141699C-R210
CGTCCGTAATGTGTTATTGAGCTAAGAGAATTACACCTCTTCAACATCACCCAGAATCAT
ATTCAAATGCTGATCAAAAGCCTACACCACAAAAACAACAACAAAAGTGAGAGTCTTAGA
ATTTAACAAGATTATGAGGTTTTCTACGCCAATTAGCTCTCGCTGAGGCTTTCGAAACTG
AAACTCAACAAAATGGGTCCAAATTTTGATATTTGTGAAAAGAATAAAAACTAGTGTTAA
ATTCAACGAATAAGGAATTCAAAAAGGAGCTCAAAATCGAAAAAGATCACATCTGGTTAC
TGGTTTCTGGAATCCGAGTGGTTAAGAACGTACGTGAAGCTTGCCGCGGAGTTCACGGTC
AGATCGGAGCTTGACATAGATCCTCTCTTCGATACTCAACCGAAT

Last Updated on 06.12.2018 16:11