Sequences from confirmation amplicon


Confirmation sequences for line SALK_142273 and hit At1g69030 are shown.
Line ID SALK_142273
Locus At1g69030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25948203
Primers used for PCR 48wj - LBb3, show primer details
   
Sequence Name 25-K118627-SALK_142273C-48wj
GenBank Accession LN485016 [GenBank]
Sequencing primer gene specific primer, 48wj: 5'-TGCTCAGACGATGCAGTAGATATT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118627-SALK_142273C-48wj
CTTCAGCCCTGGACTCTTCTTAGCTTCTTCATCTTTACTCTCTGCTTTGTTCCTAAGTTC
CTCCATGTACTTCCTTTCATATCTGATTCAAAACAAATCCTTTCCGCTTAATTCATGCAG
CTATCAAGTCTCATAAGAATGAAAGACAAGGGTGCTCAATTCACTAATCACTTCTCAGAA
ATACACAAACAACTAAGGTGTCAAGATCTTCTATATGTTTTCAGTCATGTTCCTTTGTTT
TTTATATTCTCATTTGTGAGTTACATGGTTTTCCGAGATATACTGGACCAAGCTAGAGGC
AGAATAGATGATTTGCAGCCACAAGAGAATTCACTAAGACTGTCTCTGGATCAAATCCGT
GCTGATAAGGTATAATCCATTTCCAAAGATAAGGTATAAAAACGTCCGCAATGTGTTATT
AAGTTGTCTAAGCGTCAATTTGTTTACACCACAATAAGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCG
   
Sequence Name 14-K118626-SALK_142273C-R210
GenBank Accession LN484682 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118626-SALK_142273C-R210
CACTTAATAACCATTGCGGACGTTTTTATACCTTATCTTTGGAAATGGATTATACCTTAT
CAGCACGGATTTGATCCAGAGACAGTCTTAGTGAATTCTCTTGTGGCTGCAAATCATCTA
TTCTGCCTCTAGCTTGGTCCAGTATATCTCGGAAAACCATGTAACTCACAAATGAGAATA
TAAAAAACAAAGGAACATGACTGAAAACATATAGAAGATCTTGACACCTTAGTTGTTTGT
GTATTTCTGAGAAGTGATTAGTGAATTGAGCACCCTTGTCTTTCATTCTTATGAGACTTG
ATAGCTGCATGAATTAAGCGGAAAGGATTTGTTTTGAATCAGATATGAAAGGAAGTACAT
GGAGGAACTTAGGAACAAAGCAGAGAGTAAAGATGAAGAAGCTAAGAAGAGTCCAGGGCT
TGGAAGGACAGAATCAGCTGAAAAGAACAGAATATCTACTGCTCNTTCTGAGC

Last Updated on 06.12.2018 16:11