Sequences from confirmation amplicon


Confirmation sequences for line SALK_142359c and hit At1g01440 are shown.
Line ID SALK_142359c
Locus At1g01440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:162084
Primers used for PCR 41ea - LBb3, show primer details
   
Sequence Name 72-K110924-SALK_142359C-41ea
GenBank Accession LN486053 [GenBank]
Sequencing primer gene specific primer, 41ea: 5'-TCTTAGGCACATATAGCAGCTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K110924-SALK_142359C-41ea
CGCCCGCCTGCTAGAGGATGGCAACGACGTGAATCAGTATCAGACACAGCTTGTTGAACA
TACCCACGAGCAAAAGCACCACCCATTTCAGAATCAATTTCACTAGGCCACCTCGTGTGA
ACACCACCATGTGGACTAAAGGGTCCAACTTTCTCACCGTGGTGCAAAGCCGGGCTAAAT
GGGACATCACCATACCTACTTGGCGTACCCGGAAAGAATCTAGGGTCCTGTAACTGTGAC
TCAAGTATATCAGAGCCAATGTCTACATACTGTCCTTGAGGATAGGGTGGACGAGGCGGC
ATCTGATTAGGGTAATGACTTGGTCCTTGATAAAAGTTTTGAGGTATCCTATGCACTGGG
AATCCATAAGGGTCTCTATAAGCTGGAACATGAGGTTGGTTACTCGCGGTAGGATACATT
CCATAAGGAAATGGCTCAGGGTATGGATTGTAATAAGAAGGAGGAGGTCCATGATGGGCA
TGGAAACCCATTGGTGGCTGATCAACAACAGTAGCATTATTACCTCCACCACGGAGAAGA
TGATCTTTAATAGAATCAAGCTTTCTCAAAAGCTCGGCTTGTTGTTTATCAGTGTACTGA
ATTGGGTTGTTTCTGGTGGTTGTGTTCTTAGAAGAAGAAGAATCTGAATCAGTCTCTTCC
TCCTTTCTCGAGCTCTTGACGAATCTCTGAGAACCAGGATATATTGTGGTGTAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCANGCGAAAATCCTGTTTGA
   
Sequence Name 68-K110923-SALK_142359C-R210
GenBank Accession LN485951 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K110923-SALK_142359C-R210
GTCATTTGTTTACCCCAATATATCCTGGTTCTCAGAGATTCGTCAAGAGCTCGCGAAAGG
AGGAAGAGACTGATTCAGATTCTTCTTCTTCTAAGAACACAACCACCAGAAACAACCCAA
TTCAGTACACTGATAAACAACAAGCCGAGCTTTTGAGAAAGCTTGATTCTATTAAAGATC
ATCTTCTCCGTGGTGGAGGTAATAATGCTACTGTTGTTGATCAGCCACCAATGGGTTTCC
ATGCCCATCATGGACCTCCTCCTTCTTATTACAATCCATACCCTGAGCCATTTCCTTATG
GAATGTATCCTACCGCGAGTAACCAACCTCATGTTCCAGCTTATAGAGACCCTTATGGAT
TCCCAGTGCATAGGATACCTCAAAACTTTTATCAAGGACCAAGTCATTACCCTAATCAGA
TGCCGCCTCGTCCACCCTATCCTCAAGGACAGTATGTAGACATTGGCTCTGATATACTTG
AGTCACAGTTACAGGACCCTAGATTCTTTCCGGGTACGCCAAGTAGGTATGGTGATGTCC
CATTTAGCCCGGCTTTGCACCACGGTGAGAAAGTTGGACCCTTTAGTCCACATGGTGGTG
TTCACACGAGGTGGCCTAGTGAAATTGATTCTGAAATGGGTGGTGCTTTTGCTCGTGGGT
ATGTTCAACAAGCTGTGTCTGATACTGATTCACGTCGTTGCCATCCTCTAGCAGGCGGTG
CGCCTTTTATTGCTTGTCACAGTTGCTTTGAGCTGCTATAT

Last Updated on 06.12.2018 16:11