Sequences from confirmation amplicon


Confirmation sequences for line SALK_142412 and hit At1g03050 are shown.
Line ID SALK_142412
Locus At1g03050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:708387
Primers used for PCR 24mh - LBb3, show primer details
   
Sequence Name 19-K114334-SALK_142412C-24mh
GenBank Accession LN484821 [GenBank]
Sequencing primer gene specific primer, 24mh: 5'-TCGGCTTCAGATTTAACAGATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114334-SALK_142412C-24mh
GTCTCTATAACTCATCATGAGATCAAGCTTCTTCTGTGTGATCTTCTCTATCTCTGGATA
CTCAGAAGACCGTGCGATCCCCATGTTCTTACACCAAGAGTAGAACTGATCGAGCTCTTC
GAATTGTTTCGAGACCCGGCAGAAAATGTCATAGACCTTGATCGAATCAGGAATATCTAA
CTCCATGAATCGTTCAATCAAAATCCCCATTATCTCTGTTACATCGTAATATATCTGAAA
ACTCTCCTTCACTATCGGATACAGAGCCACAATCACAACTCTGTTGTTCCTCGCATTACC
TGTTAACAAAATAACATTGGTCCTTATATAAATTTTTATCCAAGAAACAAACACACACAG
AGAAAGAAATCTTGAGACGCANGTTATTACCTGTTGGGCGACAAGCCAAGAAACGGTCAA
GAAGTTGCTGCAAATGTTGGATTCTGATGAAAATCTGCTCTGTTTTCATCTCGGCGATCG
GTTGTGACCTGACCACGATAGCCGTGGAGAGATCTGCAAACAAATTGACGCTNAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 09-K114333-SALK_142412C-R210
GenBank Accession LN484524 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114333-SALK_142412C-R210
ATCTCTCCCGGCTATCGTGGTCAGGTCACAACCGATCGCCGAGATGAAAACAGAGCAGAT
TTTCATCAGAATCCAACATTTGCAGCAACTTCTTGACCGTTTCTTGGCTTGTCGCCCAAC
AGGTAATAACTTGCGTCTCAAGATTTCTTTCTCTGTGTGTGTTTGTTTCTTGGATAAAAA
TTTATATAAGGACCAATGTTATTTTGTTAACAGGTAATGCGAGGAACAACAGAGTTGTGA
TTGTGGCTCTGTATCCGATAGTGAAGGAGAGTTTTCAGATATATTACGATGTAACAGAGA
TAATGGGGATTTTGATTGAACGATTCATGGAGTTAGATATTCCTGATTCGATCAAGGTCT
ATGACATTTTCTGCCGGGTCTCGAAACAATTCGAAGAGCTCGATCAGTTCTACTCTTGGT
GTAAGAACATGGGGATCGCACGGTCTTCTGAGTATCCNGAGATAGAGAAGATCACACAGA
AGAAGCTTGATCTCATGGATGAGTTTATAAGAGACAAATCTGCTTTAGAACACACCAAGC
AATCAAAATCTGTTAAATTGAAAGCCG

Last Updated on 06.12.2018 16:11