Sequences from confirmation amplicon


Confirmation sequences for line SALK_142729 and hit At4g14760 are shown.
Line ID SALK_142729
Locus At4g14760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8478380
Primers used for PCR CE25 - R204, show primer details
   
Sequence Name 70-K106742-SALK_142729C-R210
GenBank Accession LN485996 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K106742-SALK_142729C-R210
ATTCTTCTGCTCTAGATCTTCTATAAGCTTTTGCAAGATGAAGATCCCGATTTGTTTGTT
TACACTCTGTCAAGCTCATCCTCGTACTCTCTTTTCCTGGAACGACACTCTTCTCGAAGA
AAGCTCACATTCTTCTGAAGATCTGCTAATCTGCTTTCAGTAGATCGTTTGTAATTAGCA
CTCTCCTGCTTCTCAGCGGCAAGTGAGACCTGTAACTCTTCTACTTGATGGCTTTTGAGC
TTATTATCTCTCTGTAAATCAGTATACCTTACTTCCAGTTCTGTATACTTCTTCTCCAAG
ACTCCTAGCTTCTCCTCAACCTTACACAACTGAGAGACAAGAGATTCTCTTTCCTTCATA
AGCTCAGACTTATCATTTTTGAGAAACTGGAAGATATCATCAAAGCACT
   
Sequence Name 86-K106743-SALK_142729C-CE25
GenBank Accession LN486292 [GenBank]
Sequencing primer gene specific primer, CE25: 5'-AGTGCTTTGATGATTTCTTCCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K106743-SALK_142729C-CE25
GTCTTGGAGAAGAAGTATACAGAACTGGAAGTAACGCATACTGATTTACAGAGAGATAAT
AAGCTCAAAAGCCATCAAGTAGAAGAGTTACAGGTCTCACTTGCCGCTGAGACGCAGGAG
AGTGCTAATTACAAACGATCTACTGAAAGTAGATTAGCAGATCTTCAGAAGAATGTGAGC
CTTCTTCGAGAAGATTGTCGTTCCAGGAAAAGAGAGTACGAGGACCAGC
Primers used for PCR CE26 - LBb3, show primer details
   
Sequence Name 77-K118526-SALK_142729C-CE26
GenBank Accession LN486181 [GenBank]
Sequencing primer gene specific primer, CE26: 5'-GTCCAAGTAGAGATAAAAGCACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K118526-SALK_142729C-CE26
TTCAGCACTGGAGAGTGAACCTTTTAGGCTATCAATCTCTCCCAAAGCACGTGAAACTGA
AATTTGGTCTTTTGTAATCTTCTGTTCAGTCTTGCAATCTGCTTCAACTTGAAGTGCCTT
TATCACCTGATAGATTGCACCTCTCAAGCTATCAATCTCATCCAAAAATATTTCTGCTTC
CATCTGCTGCTCGAGATTCTCACTTTCCAGCTCAGCAATGAGTTTCTCGGAAAATTCTGA
TGCTTCAACATGCTTCTGACACTTAATAACACATTATATTGCAGGATATATTGTGGTGTA
AACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTG
AGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAT
GGTGGTTCCGAATGGGGCAAAAT
   
Sequence Name 67-K118525-SALK_142729C-R210
GenBank Accession LN485942 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118525-SALK_142729C-R210
CCACAATATATCCTGCAATATAATGTGTTATTAAGTGTCAGAAGCATGTTGAAGCATCAG
AATTTTCCGAGAAACTCATTGCTGAGCTGGAAAGTGAGAATCTCGAGCAGCAGATGGAAG
CAGAAATATTTTTGGATGAGATTGATAGCTTGAGAGGTGCAATCTATCAGGTGATAAAGG
CACTTCAAGTTGAAGCAGATTGCAAGACTGAACAGAAGATTACAAAAGACCAAATTTCAG
TTTCACGTGCTTTGGGAGAGATTGATAGCCTAAAAGGTTCACTCTCCAGTGCTGAATATG
AGATGCATCGACTTGTAGTTGAGAACTCGGTGCTTTTATTCTTACTTGGAC

Last Updated on 06.12.2018 16:11