Sequences from confirmation amplicon


Confirmation sequences for line SALK_142747c and hit At1g50890 are shown.
Line ID SALK_142747c
Locus At1g50890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18864076
Primers used for PCR 65cs - LBb3, show primer details
   
Sequence Name 18-K111273-SALK_142747C-65cs
GenBank Accession LN484776 [GenBank]
Sequencing primer gene specific primer, 65cs: 5'-GGCATGTCGTTTTGATAAGGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K111273-SALK_142747C-65cs
TTCTGGTAAGGATCATTGGCTAATATCTATCCAAGGTTAGAAATAAGATTGATACCAAGA
AAAGAAATGCAGATTACTCTTAATGGTTATTAAAACATTGCTATTTCCATTCTCTTTCCT
TGTCTTCAGCACCTTATGTATACGCTGATCGTGATAGCTGTGTTATAATTTTCTGATCAA
CTAAACAGAGCCAAAGGTATATAAGCTAGATTGAAAATAAAAGCTTGGATAGCAGTGGCG
GATATTTGTTATACACTGTGATTTTCGATGAGCTGAAAGAATTTGCTTAGGCATGTTTTG
CTTTGCTAGCATGTAAAAACGATAATTTAGTTTGACTGAGTTATATCCATATCACCATTG
CTTAAGAAGGGAACTGCTAGACTGGAATTACATTGTTAATTTTTCCGACATTGTTTTTGC
ATCATTCTGAAAAGTGGCTACATTTTCTTTGGGTTGCAGATAAAACCTGTTAGAGAAAGC
TTGTCTGAAGCACTAAACGTCTGGAAGAACATTGCTGGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAACGGGCACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 02-K111272-SALK_142747C-R210
GenBank Accession LN484303 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K111272-SALK_142747C-R210
TCTTCAGACGTTTAGTGCTTCAGACAGCTTTCTCTAACAGGTTTTATCTGCAACCCAAAG
AAAATGTAGCCACTTTTCAGAATGATGCAAAAACAATGTCGGAAAAATTAACAATGTAAT
TCCAGTCTAGCAGTTCCCTTCTTAAGCAATGGTGATATGGATATAACTCAGTCAAACTAA
ATTATCGTTTTTACATGCTAGCAAAGCAAAACATGCCTAAGCAAATTCTTTCAGCTCATC
GAAAATCACAGTGTATAACAAATATCCGCCACTGCTATCCAAGCTTTTATTTTCAATCTA
GCTTATATACCTTTGGCTCTGTTTAGTTGATCAGAAAATTATAACACAGCTATCACGATC
AGCGTATACATAAGGTGCTGAAGACAAGGAAAGAGAATGGAAATAGCAATGTTTTAATAA
CCATTAAGAGTAATCTGCATTTCTTTTCTTGGTATCAATCTTATTTCTAACCTTGGATAG
ATATTAGCCAATGATTCCTCTACCAGAAATATCTCAAGAAAATATCATAGCTATTAGTAC
CTTATCAAACGNACATGC

Last Updated on 06.12.2018 16:11