Sequences from confirmation amplicon


Confirmation sequences for line SALK_143488c and hit At1g01350 are shown.
Line ID SALK_143488c
Locus At1g01350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:137577
Primers used for PCR 44ux - LBb3, show primer details
   
Sequence Name 32-K117668-SALK_143488C-R210
GenBank Accession LN485175 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117668-SALK_143488C-R210
CATTTGTTGAGCAATTTGTCTAGCGTCAATGTGAATGGGCTTCTTCCCACTCTTTCTCTA
TCTGCCATCCTGGTTTGTAATCCCCACGGTCATGCAAGAACTTACACGAATCTCCATATC
CACAGTATCCGGTTTCTTTGTAATCCTTGCAAATGTCTGGCTGGTAATCGAATCTAGCCG
ACACTCTTATGTGAGCAGAAGCTCTTAATGGCCCGTGTGAGCCTCCAGCTTTCTCGCTCG
AGATTGTTTGTTCTCTTCTAAACCCAGCTTTGTGATCTGTATATCCATGAATTCCTTTGT
ACAGCTTCTCATCAGAAGCCTTTTTCTTATTCCCTTTCAA
   
Sequence Name 69-K117669-SALK_143488C-44ux
GenBank Accession LN485987 [GenBank]
Sequencing primer gene specific primer, 44ux: 5'-TTGAAAGGGAATAAGAAAAAGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K117669-SALK_143488C-44ux
GATATACGATCACAAAGCTGGGTTTAGAAGAGAACAAACAATCTCGAGCGAGAAAGCTGG
AGGCTCACACGGGCCATTAAGAGCTTCTGCTCACATAAGAGTGTCGGCTAGATTCGATTA
CCAGCCAGACATTTGCAAGGATTACAAAGAAACCGGATACTGTGGATATGGAGATTCGTG
TAAGTTCTTGCATGACCGTGGGGATTACAAACCAGGATGGCAGATAGAGAAAGAGTGGGA
AGAAGCCCATTCACATTGACGCTTAGACAAATTGCGTCAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATC

Last Updated on 06.12.2018 16:11