Sequences from confirmation amplicon


Confirmation sequences for line SALK_143623c and hit At2g41420 are shown.
Line ID SALK_143623c
Locus At2g41420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17267385
Primers used for PCR 15ew - LBb3, show primer details
   
Sequence Name 22-K111285-SALK_143623C-15ew
GenBank Accession LN484913 [GenBank]
Sequencing primer gene specific primer, 15ew: 5'-ATCATTGAGATGTGACCATACGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K111285-SALK_143623C-15ew
TTAGATCAGCNATAACTATGGCATAGAATAGTGTCTCCCACCAATTTACATTCCCATAAT
CAAATACAAACTAAGGCAGAACGTCGATAGACAATAGTTAAAATGTTATGAACCGAAGAA
CATTACACATAAAACGAAAGAGAATCATCGAGAGATTCATTTTTAATGCCCCACAAATCA
ATTAAGGTTTTTGTTTAACCAAAACCCTCTTACAAATAAATCCCGAAGCTTTATGCAGAG
AGAGAGAGACTCCAATCAGAAGCAAGCATCCAAGAGACAGCAACAACACAGAGCAGCAAG
ACTGCAAAAGCAAAAACAGTGTTTACATTTCACAATCAGTATTAAAAACAAATCCAGTTG
CTTTAAGACATGCAATGTAGTAAGCAGTAGCTAGATCATGCAAACAATCAAATCCTAAAA
GATGCACATTCATCAAAAACAACATCCGATTCACACTGTTAAGTAAAGCCATCAAAGAAC
AACAATTATCCTGTATTCAGTGAAGGAACAGAGAACACAAAAAGGATCCATCTTGCTAAG
CCAATTTACTTATCAAAACTTGTACAAGTATAGTTTCAAGTACTCATAAACAGTTAAACA
CTACCAAACCAATAACGACAAGAACACAATCACAATTCTAGTTTCTTTAAAGCATGCAAT
GTTGTAGTAACCAGTAGCTAGATCATGCAAACATGTTAAATCCTAAAAGATGCAAATTCA
TTAAACACAACAACAATTGTCCTGTGATCAGTGAATGAACAGAGATCACAAAAAGTCAAC
CTCTACAGAGGAATATTCTAGCTAAAACCACATGACCTTTTGTTCAAAGAATCTCTAGAA
GAAATATTTAAAATAAGGAATATACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTT
   
Sequence Name 11-K111284-SALK_143623C-R210
GenBank Accession LN484581 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K111284-SALK_143623C-R210
ATTTATATTTCTTCAGAGATTCTTTGAACAAAAGGTCATGTGGTTTTAGCTAGAATATTC
CTCTGTAGAGGTTGACTTTTTGTGATCTCTGTTCATTCACTGATCACAGGACAATTGTTG
TTGTGTTTAATGAATTTGCATCTTTTAGGATTTAACATGTTTGCATGATCTAGCTACTGG
TTACTACAACATTGCATGCTTTAAAGAAACTAGAATTGTGATTGTGTTCTTGTCGTTATT
GGTTTGGTAGTGTTTAACTGTTTATGAGTACTTGAAACTATACTTGTACAAGTTTTGATA
AGTAAATTGGCTTAGCAAGATGGATCCTTTTTGTGTTCTCTGTTCCTTCACTGAATACAG
GATAATTGTTGTTCTTTGATGGCTTTACTTAACAGTGTGAATCGGATGTTGTTTTTGATG
AATGTGCATCTTTTAGGATTTGATTGTTTGCATGATCTAGCTACTGCTTACTACATTGCA
TGTCTTAAAGCAACTGGATTTGTTTTTAATACTGATTGTGAAATGTAAACACTGTTTTTG
CTTTTGCAGTCTTGCTGCTCTGTGTTGTTGCTGTCTCTTGGATGCTTGCTTCTGATTGGA
GTCTCTCTCTCTCTGCATAAAGCTTCGGGATTTAATTTGTAAGAGGGTTTTTG

Last Updated on 06.12.2018 16:11