Sequences from confirmation amplicon


Confirmation sequences for line SALK_143628c and hit At3g03780 are shown.
Line ID SALK_143628c
Locus At3g03780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:960448
Primers used for PCR 23gq - LBb3, show primer details
   
Sequence Name 03-K112347-SALK_143628C-R210
GenBank Accession LN484338 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112347-SALK_143628C-R210
TTTGACTCCCATTGAGAACTCTCGTTCAGACGAGAAGCTTCTCTCAGTGTTCCCTGTAGG
AGTGAAGTACGGTGCAGGAATCGGTCCTGGTGTTTACGACATTCACTCTCCGAGAATACC
ATCCACAGATGAAATTGCAGACAGGATCAACAAGATGCTTGCGGTTCTTGAGCAGAACAT
CTTGTGGGTTAACCCTGACTGTGGTCTGAAGACAAGGAAGTACACTGAGGTTAAACCAGC
ACTTAAAGCCATGGTTGACGCGGCTAAGCTTATCCGCTCCCAGCTCGGTAGTGCCAAGTG
AAGAGCTTGAAGATATTATTTCTATATTCCGGGATTTTTCTACGTGGTTTGTGT
   
Sequence Name 12-K112348-SALK_143628C-23gq
GenBank Accession LN484613 [GenBank]
Sequencing primer gene specific primer, 23gq: 5'-AACACAAACCACGTAGAAAAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K112348-SALK_143628C-23gq
GCTCTTCACTTGGCACTCCGAGCTGGGAGCGGATAAGCTTAGCCGCGTCAACCATGGCTT
TAAGTGCTGGTTTAACCTCAGTGTACTTCCTTGTCTTCAGACCACAGTCAGGGTTAACCC
ACAAGATGTTCTGCTCAAGAACCGCAAGCATCTTGTTGATCCTGTCTGCAATTTCATCTG
TGGATGGTATTCTCGGAGAGTGAATGTCGTAAACACCAGGACCGATTCCTGCACCGTACT
TCACTCCTTCACGGAACACTGAGAGAAGCTTCTCGTCTGAACGAGAGTTCTCAATGGTGA
TGTCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
TGGTGGTTCCGAAATGNGCAAAA

Last Updated on 06.12.2018 16:11