Sequences from confirmation amplicon


Confirmation sequences for line SALK_143876c and hit At2g39690 are shown.
Line ID SALK_143876c
Locus At2g39690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16540825
Primers used for PCR 41oh - LBb3, show primer details
   
Sequence Name 25-K115143-SALK_143876C-41oh
GenBank Accession LN485007 [GenBank]
Sequencing primer gene specific primer, 41oh: 5'-CACGATGGCTAGTTCTTGTATGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115143-SALK_143876C-41oh
CATTGTTTGATCTTAAGAAATAATTAACATTATGTGAGGTTATGTTTATTGGTATTGCCT
GAGGTGGAAGAAACAATGAGGAGAGACTTGGGAGAGAAACAATAGAGCCATCAAAAGCAA
CTCGTAAAGTTCTAGTCACTGCTTCTTCTTCCTCTAGTCTCTCTTTAAGATGCATCACCT
ACATAAACACAAACATTCAAATTATTGGTATAGAGATTTTGAGTGAGGAACAGAGGTGCT
TACTCCTTCCTCTAAGTCATGTCTCTTGTTACTATGTAGCTGTGATGAATCTTCCATGAG
TAGTTCCTCAAATTTCATGAGTTTCAGAGAAACATAAGAAGAAGGAAACGGGTCTAAGTA
GAGAAAGAGAATGATTAGAGTGAAACGTGGGAGCCATTAAAAGGGTCGGTTCGGAGTATT
AAAACAGTTATTGACTTTTGTTTTTTGGTCACTTGAGGTTGTTAAGAAGGGATAATGTAA
GTCACATGAGACTTGCCGGAATAACGGCGGATCAGGATATATTGTGGTGTAAACAAATTG
ACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAANATCCTGTTTGATGGTGGTTCC
G
   
Sequence Name 09-K115142-SALK_143876C-R210
GenBank Accession LN484527 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115142-SALK_143876C-R210
TTTGTTTCCCACAATATATCCTGATCCGCCGTTATTCCGGCAAGTCTCATGTGACTTACA
TTATCCCTTCTTAACAACCTCAAGTGACCAAAAAACAAAAGTCAATAACTGTTTTAATAC
TCCGAACCGACCCTTTTAATGGCTCCCACGTTTCACTCTAATCATTCTCTTTCTCTACTT
AGACCCGTTTCCTTCTTCTTATGTTTCTCTGAAACTCATGAAATTTGAGGAACTACTCAT
GGAAGATTCATCACAGCTACATAGTAACAAGAGACATGACTTAGAGGAAGGAGTAAGCAC
CTCTGTTCCTCACTCAAAATCTCTATACCAATAATTTGAATGTTTGTGTTTATGTAGGTG
ATGCATCTTAAAGAGAGACTAGAGGAAGAAGAAGCAGTGACTAGAACTTTACGAGTTGCT
TTTGATGGCTCTATTGTTTCTCTCCCAAGTCTCTCCTCATTGTTTCTTCCACCTCAGGCA
ATACCAATAAACATAACCTCACATAATGTTAATTATTTCTTAATGATCAAACAATGATGA
GATTTTATTGTTCAACAGTTTTCTGAGCTCAGACNAGAACTA

Last Updated on 06.12.2018 16:11