Sequences from confirmation amplicon


Confirmation sequences for line SALK_144987 and hit At1g79210 are shown.
Line ID SALK_144987
Locus At1g79210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29796320
Primers used for PCR 79hw - LBb3, show primer details
   
Sequence Name 22-K113191-SALK_144987C-79hw
GenBank Accession LN484917 [GenBank]
Sequencing primer gene specific primer, 79hw: 5'-TTGTAATGTTTGACGGATTGTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K113191-SALK_144987C-79hw
TAATGCTTTTCTTTCCGTTTTTTCTTCAGAAAGAGGGAATTCCAGAGCTATCAATGGAAG
ACTACTTCTCGAAAAACAACGAGTTTGCTACATGGCTGAAAGAAGAAAAACGTACTTACT
TTAATGATCTCACGACCGAAGCTGCACGAGGACTGTTCTCACGATTTGTCAAGAGATGGA
ACAGAGGAAAACTCGAGTCTCGATACTACGAGGGAATCTCCACTGCCCCACGAACAGCTC
ACGACTGGATGATCAAGCGTAGGTGAAAAAAAGCAAAAGCAATACCTTCCTCTTCTGTGG
TTCGCCGGTTACCTCTCTTCTTGCTTCTTGTACACGTGAAGGTTGATTGCATCAGAGATA
TATTTCTATGTAGTTTCTCTACTTAGAACTTCTTGTTGCTGAACTAGTTAGATAGATTGT
CGTGGAGAGATATGTAGTCGGTTTTCAGAGTAAGACAAACTTTACATATCCAAGGTCTTA
ACCAAAGTTGCCGAAGAACTATCTGATGAATCCTTATGTAACATTGAGAATTTGATATCC
GGTTAAGGGAGAAATAGACCGTTTTGATTGAAAGTTGGTCTTGTACAGTATGTAAGTTCC
TACTGGCATTTACAACACAGCGTATATGTAGCTTTGGCTAAGTAATTTGTGTGCATGAAA
CTTGGTTATATGAAACTGAATTTTCACAACAGACGTTTAAATGAGTGAAAAAACATATTC
TAAATTATTGGGTCACGAGTCTCCTTAACAAATTCCAAAAGTTTTACAAAAGCAAGAACC
ATNCACTCTGAGAGTATACTGAGAT
   
Sequence Name 11-K113190-SALK_144987C-R210
GenBank Accession LN484587 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K113190-SALK_144987C-R210
ATTTGTTTTTCAAAAGTTGTCAGAAATCGATGATTACTTGGCTGAAGTCGAGTAATTCTT
TTCCTTAACCGTGCAACCAACCTGATTTGAATCTCAGTATACTCTCAGAGTTGATGGTTC
TTGCTTTTGTAAAACTTTTGGAATTTGTTAAGGAGACTCGTGACCCAATAATTTAGAATA
TGTTTTTTCACTCATTTAAACGTCTGTTGTGAAAATTCAGTTTCATATAACCAAGTTTCA
TGCACACAAATTACTTAGCCAAAGCTACATATACGCTGTGTTGTAAATGCCAGTAGGAAC
TTACATACTGTACAAGACCAACTTTCAATCAAAACGGTCTATTTCTCCCTTAACCGGATA
TCAAATTCTCAATGTTACATAAGGATTCATCAGATAGTTCTTCGGCAACTTTGGTTAAGA
CCTTGGATATGTAAAGTTTGTCTTACTCTGAAAACCGACTACATATCTCTCCACGACAAT
CTATCTAACTAGTTCAGCAACAAGAAGTTCTAAGTAGAGAAACTACATAGAAATATATCT
CTGATGCAATCAACCTTCACGTGTACAAGAAGCAAGAAGAGAGGTAACCGGCGAACCACA
GAAGAGGAAGGTATTGCTTTTGCTTTTTTTCACCTACGCTTGATCATCCAGTCGTGAGCT
GTTCGTGGGGCAATGGAGATTCCCTCGTAGTATCGAGACTCGAGTTTTCCTCTGTTCCAT
CTCTTGACAAATCGTGAGAACCGTCCTCGCGCAGCTTCGGTCGTGAGATTATTAAAGTAA
GTACGTTCTTCTTTCTTTCAGCCATGTAGCAAAACTCCGTGGTTTTTCGA

Last Updated on 06.12.2018 16:11