Sequences from confirmation amplicon


Confirmation sequences for line SALK_147032c and hit At3g19380 are shown.
Line ID SALK_147032c
Locus At3g19380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6714620
Primers used for PCR 57cy - LBb3, show primer details
   
Sequence Name 45-K109566-SALK_147032C-57cy
GenBank Accession LN485388 [GenBank]
Sequencing primer gene specific primer, 57cy: 5'-GCCCAGTTTGTTTATTGTTCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K109566-SALK_147032C-57cy
GGCAAAGGACTTTTGGGGTCCTGTGGAATAGAGACTAATCGAAATGTTAACAAATGCTTT
AACACCATTCAACAAGAAAAAACTCAAGTTGCACCCAAAAAATAGAAAAAGGAGACACTT
AAAAGAAGATAAATTAACAAGAGCTCTACAACACAAAGTACAAACTACCATAATGACGTT
CACTAGAAGCTTACATGTCGATACCAAATTTGCCAACATGTCGATACCAAAATTTACCTG
TAAAAATGCAGAAAAAAGTTGACTTACCTTTAGACTTTAAACTTTAAAAAGCCTTTAAGC
ATATCTTCCATTTGCTTCTTTAACAACTTTTCCCAACTCAAAAGGCCAATCAAATAACAA
AATAATTACTTTACATACATACTGAAAAAAAAAAAAACTAATTCAGGGCCATCAAAAAGC
TCCGGAAAATAACCTTTTCTTCTCCACATCTTTCCTTCAAATACCCCCAAAAAATGACCT
TTTTTTTTCCTTCTAATTAAAAAAAAAACTTTTTTTAAAAAATACCGGGAAAAAAACAAG
GTTTTGAAAAAAAAAATTCGAAAATTCAAAAGGGGCCCCTTGGGTGGGGGCACCCAAAGT
   
Sequence Name 36-K109565-SALK_147032C-R210
GenBank Accession LN485232 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K109565-SALK_147032C-R210
GCGTCATTTGTTACACCACAATATATCCTGACTACATTCCTTCGCTAACTCCGATGACTT
TGGTTGCAGCAGCCAAGTGGTCCCTTTTTGATTTCTCGATTTTTTTTTTTCAAAACATTG
TTTTTTCACAGTAATTATTAAAAAAATGTTTTTTTTTTAATTAAAAGGAAAAATAAGGGC
CATTTTTTGGGGGTATTTGAGGGAAAAATGTGAAAAAAAAAAAATAATATCCAAAAGCTT
TTTGAGGACCATGAATTAGTTTTTTTTTTTTCCAATAGGTTGGAAAAAAAATTTTTTGGT
TTTTGAAGGGCCCTTTGGAGTGGGAAAAATTTTTAAAAAAACCAAAGGGAAAATTTCCTA
AAGGGTTTTTAAAATTTAAAAGCCAAAGGGAAAGCCACTTTTTTCCGCCTTTTTACCGGG
AAAATTTGGGAACCAACTGGGGGGAAAATTGGGACCCCACTGGAAACCTCCTGGGAACCC
CCTTTAGGGGAGTTGGCCCTTGGGGTGGAAAACCCCTGGAAAATTAACCTCCTTTAAGGG
GCCCCCTTTTCCATTTTTTGGGGGGCACCTGGGTTTTTTCCTGGGAAAAGGGGGTAAAAC
CATTTGTAAACATTCCAATAAGCCCCTTTTTAAGGGCCCCCAAAATACCTTTAAAGGGGG
GCAACTTGCAAAAAATA

Last Updated on 06.12.2018 16:11