Sequences from confirmation amplicon


Confirmation sequences for line SALK_147280c and hit At1g16500 are shown.
Line ID SALK_147280c
Locus At1g16500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5639814
Primers used for PCR 48um - LBb3, show primer details
   
Sequence Name 91-K117830-SALK_147280C-48um
GenBank Accession LN486343 [GenBank]
Sequencing primer gene specific primer, 48um: 5'-CTAACCCGTGACTTAAGTTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K117830-SALK_147280C-48um
GGTTCTTTAGATGCGGAATAATACCAGTACAGTTGAAGTAAATATAATGAAATATGTTGA
CCAAATAATATAAGAGTATAATTTGGTTACCTCTTACTCTCTAGTAGTATCTAATTAATT
AGAGCGTTTTTCAAATAATTAAAGATCAACTATTCATTCATCAGAGAAAATCCATTACAT
AACAATTTACAAAATAACATCCATCTCTTCCTTTCTGCATTTAATTTCTTTTCAATGAAA
CCCCCTGATTTTATCTATCATTTACAGAAATCGAACCTACAAAATAAAACCATTCAATTC
TATCCCCCAAAGTTTTTTTCACATCGAAATGTTAAGATCAATCTTAGGCTTCTTCATGGC
AGGCAAAGGAACAAACGAATGACACCTCGGGCATTTCGGATTGTTCTTTGCCACAAACAC
GTACGATAAGCACCCTGGACATCCAGCCGCCACCTGAGACGCCGCGACGGTGGCAGAAGC
GGTTCCATCGTAAACGCCGTTTAAACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 74-K117829-SALK_147280C-R210
GenBank Accession LN486117 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K117829-SALK_147280C-R210
TTTGTTAACGGCGTTACGATGGAACCGCTTCTGCCACCGTCGCGGCGTCTCAGGTGGCGG
CTGGATGTCCAGGGTGCTTATCGTACGTGTTTGTGGCAAAGAACAATCCGAAATGCCCGA
GGTGTCATTCGTTTGTTCCTTTGCCTGCCATGAAGAAGCCTAAGATTGATCTTAACATTT
CGATGTGAAAAAAACTTTGGGGGATAGAATTGAATGGTTTTATTTTGTAGGTTCGATTTC
TGTAAATGATAGATAAAATCAGGGGGTTTCATTGAAAAGAAATTAAATGCAGAAAGGAAG
AGATGGATGTTATTTTGTAAATTGTTATGTAATGGATTTTCTCTGATGAATGAATAGTTG
ATCTTTAATTATTTGAAAAACGCTCTAATTAATTAGATACTACTAGAGAGTAAGAGGTAA
CCAAATTATACTCTTATATTATTTGGTCAACATATTTCATTATATTTACTTCAACTGTAC
TGGTATTTATTCCGCATCTTAAAGAACCCAATGTTTTTATTTGTCATATTAAGGAAGCAA
CTTAAGTCACCGGGTTA

Last Updated on 06.12.2018 16:11