Sequences from confirmation amplicon


Confirmation sequences for line SALK_147407 and hit At1g52550 are shown.
Line ID SALK_147407
Locus At1g52550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19573162
Primers used for PCR 06cq - LBb3, show primer details
   
Sequence Name 14-K108551-SALK_147407C-06cq
GenBank Accession LN484661 [GenBank]
Sequencing primer gene specific primer, 06cq: 5'-TGAAGGTGGTTTAACTGCTATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K108551-SALK_147407C-06cq
TCTCAGATCGATACTGTTCATTGCTAAAGTTTTGATCTTTGTGACAATTTATAGACTTTT
ACTCTTGTTAAGCATTAAGGATTTATAAATCTCCTCCTTTTACAACTTGTGAATGTCTTC
CAAAAGTTCCACCATTTTCTTTTCCCTACAGGTTTAGTCATTGGTTGCAATACGAGAGCA
ATCTTCTTAACAATTTGGATTCATAGTTGTTGTTTGGTATCACATTTTGTGTAGGTCTTG
TGGGAGTAGCGTCGATGATTCAAGACCGTGTAAAGATCTTTCTTTCAGTACTAATTTGGA
TCTCTCTCTTCTTTTGGGCTTCGGCTTTACAAGGAAGAGGCAGCAAAGGTAATGGTAAAG
GGAAGAAGGGTTCACGCTTCACATAATGTGGTGAAACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 04-K108550-SALK_147407C-R210
GenBank Accession LN484359 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K108550-SALK_147407C-R210
GTCATTTGTTTCACCCATTATGTGAAGCGTGAACCCTTCTTCCCTTTACCATTACCTTTG
CTGCCTCTTCCTTGTAAAGCCGAAGCCCAAAAGAAGAGAGAGATCCAAATTAGTACTGAA
AGAAAGATCTTTACACGGTCTTGAATCATCGACGCTACTCCCACAAGACCTACACAAAAT
GTGATACCAAACAACAACTATGAATCCAAATTGTTAAGAAGATTGCTCTCGTATTGCAAC
CAATGACTAAACCTGTAGGGAAAAGAAAATGGTGGAACTTTTGGAAGACATTCACAAGTT
GTAAAAGGAGGAGATTTATAAATCCTTAATGCTTAACAAGAGTAAAAGTCTATAAATTGT
CACAAAGATCAAAACTTTAGCAATGAACAGTAATCGATCTTGAGGACTAAGATTAACATC
TTTATCTACGAATCAATAGCAGTTAAACCACCTTC
Primers used for PCR SC04 - LBb3, show primer details
   
Sequence Name 15-K108551-SALK_147407C-SC04
GenBank Accession LN484690 [GenBank]
Sequencing primer gene specific primer, SC04: 5'-TCAAGATCGATTACTGTTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K108551-SALK_147407C-SC04
ATTATGACTTTACTCTTGTTAGCATTAAGGATTTTAAATCTCCTCCTTTTACAACTTGTG
AATGTCTTCCAAAAGTTCCACCATTTTCTTTTCCCTACAGGTTTAGTCATTGGTTGCAAT
ACGAGAGCAATCTTCTTAACAATTTGGATTCATAGTTGTTGTTTGGTATCACATTTTGTG
TAGGTCTTGTGGGAGTAGCGTCGATGATTCAAGACCGTGTAAAGATCTTTCTTTCAGTAC
TAATTTGGATCTCTCTCTTCTTTTGGGCTTCGGCTTTACAAGGAAGAGGCAGCAAAGGTA
ATGGTAAAGGGAAGAAGGGTTCACGCTTCACATAATGTGGTGAAACAAATTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGG
CAAAA
   
Sequence Name 05-K108550-SALK_147407C-r210
GenBank Accession LN484359 [GenBank]
Sequencing primer gene specific primer, r210: unknown
Sequence (A. th genome BLAST matches underlined) 
>05-K108550-SALK_147407C-r210
GTCATTTGTTTCACCCATTATGTGAAGCGTGAACCCTTCTTCCCTTTACCATTACCTTTG
CTGCCTCTTCCTTGTAAAGCCGAAGCCCAAAAGAAGAGAGAGATCCAAATTAGTACTGAA
AGAAAGATCTTTACACGGTCTTGAATCATCGACGCTACTCCCACAAGACCTACACAAAAT
GTGATACCAAACAACAACTATGAATCCAAATTGTTAAGAAGATTGCTCTCGTATTGCAAC
CAATGACTAAACCTGTAGGGAAAAGAAAATGGTGGAACTTTTGGAAGACATTCACAAGTT
GTAAAAGGAGGAGATTTATAAATCCTTAATGCTTAACAAGAGTAAAAGTCTATAAATTGT
CACAAAGATCAAAACTTTAGCAATGAACAGTAATCGATCTTGA
Primers used for PCR 16wp - LBb3, show primer details
   
Sequence Name 47-K118528-SALK_147407C-16wp
GenBank Accession LN485446 [GenBank]
Sequencing primer gene specific primer, 16wp: 5'-ACCTCACCTTGGTGAATCTGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118528-SALK_147407C-16wp
GTTCAAATACTAGNTTTTTTTTATTGTGTATGAAGAAGATCTCTAGATATGGAAATTTAT
TTGCTATAATCAGAACAAATATAAATTAAACATAGCATAAAATCTTAATTTAGATAGATT
GTTAAATTACTCAGTGTGGTCAGAACAAAATGTTAGGCTCTTATCATAAATTTCTGTTTC
TAAACTTACTACCAGCCTTTAACCAAAACAGGTCCAACTAATCATGAGCTGGTTAATTCA
TTGTCCCAGTATTGAATAAGGTTTACCTAAATCCAGTTATTTCTTTTTTCATATCATGAA
TAAACAACCACTGTTTTAAATATAAATCAAATAATAATTAAATACAACTTTGGAAATATT
ATATTATATAGAGAGGCCCAATAAAAGAGTATGTTGAAGCCCAAACAAGTTATATAACAT
ATCTAAAGGCCCATTTTATACAACAAACATTCAAATCAAAGAAACGGTGCGGTTTACCGT
TTGGCTGAATCAAGCACACACCAAACACATCTAGTCATTTACAATCTGAAACAGCTTTCA
ATAAACAATGATATTGATTTGGCATTTTGACTTAAATGCAAACTACTTTAGTTTCATCAC
AGTAGAGTAATCTCTATAGCTCTAAGCTAGAGATATCGTAAGAAGAGAACAATCTAGTTT
CTAGTATAAAACTGTAGTCTGACAAGAAATTTGCTTTACTTGGAAGAATATTTCCTCTGT
ATATATACACAAACGTGTAGAGGAGTTTTCATCAATCATCATCATCATCACCACACCAAT
CTATTGACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACT
GGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTTCACGCCTGG
CCCTG
   
Sequence Name 45-K118527-SALK_147407C-R210
GenBank Accession LN485403 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118527-SALK_147407C-R210
ATGATGATGATGATTGATGAAAACTCCTCTACACGNTTTGTGTATATATACAGAGGAAAT
ATTCTTCCAAGTAAAGCAAATTTCTTGTCAGACTACAGTTTTATACTAGAAACTAGATTG
TTCTCTTCTTACGATATCTCTAGCTTAGAGCTATAGAGATTACTCTACTGTGATGAAACT
AAAGTAGTTTGCATTTAAGTCAAAATGCCAAATCAATATCATTGTTTATTGAAAGCTGTT
TCAGATTGTAAATGACTAGATGTGTTTGGTGTGTGCTTGATTCAGCCAAACGGTAAACCG
CACCGTTTCTTTGATTTGAATGTTTGTTGTATAAAATGGGCCTTTAGATATGTTATATAA
CTTGTTTGGGCTTCAACATACTCTTTTATTGGGCCTCTCTATATAATATAATATTTCCAA
AGTTGTATTTAATTATTATTTGATTTATATTTAAAACAGTGGTTGTTTATTCATGATATG
AAAAAAGAAATAACTGGATTTAGGTAAACCTTATTCAATACTGGGACAATGAATTAACCA
GCTCATGATTAGTTGGACCTGTTTTGGTTAAAGGCTGGTAGTAAGTTTAGAAACAGAAAT
TTATGATAAGAGCCTAACATTTTGTTCTGACCACACTGAGTAATTTAACAATCTATCTAA
ATTAAGATTTTATGCTATGTTTAATTTATATTTGTTCTGATTATAGCAAATAAATTTCCA
TATCTAGAGATCTTCTTCATACACAATAAAAAAAACTAGTATTTGAACTTTTGTATGTAT
TAAAAATTGCAAGATTTTGTAAGTACAGATTCACA
Primers used for PCR 01cb - LBb3, show primer details
   
Sequence Name 84-K118526-SALK_147407C-01cb
GenBank Accession LN486278 [GenBank]
Sequencing primer gene specific primer, 01cb: 5'-AGCACACACCAAACACATCTAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K118526-SALK_147407C-01cb
ATATTGATTTGGCATTTTGACTTAATGCAAACTACTTTAGTTTCATCACAGTAGAGTAAT
CTCTATAGCTCTAAGCTAGAGATATCGTAAGAAGAGAACAATCTAGTTTCTAGTATAAAA
CTGTAGTCTGACAAGAAATTTGCTTTACTTGGAAGAATATTTCCTCTGTATATATACACA
AACGTGTAGAGGAGTTTTCATCAATCATCATCATCATCACCACACCAATCTATTGACAAA
TTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTT
TCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAG
TTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
TCCGAATGCGGCAAAA
   
Sequence Name 74-K118525-SALK_147407C-R210
GenBank Accession LN486120 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K118525-SALK_147407C-R210
TCATAGATTGGTGTGGTGATGATGATGATGATTGATGAAAACTCCTCTACACGTTTGTGT
ATATATACAGAGGAAATATTCTTCCAAGTAAAGCAAATTTCTTGTCAGACTACAGTTTTA
TACTAGAAACTAGATTGTTCTCTTCTTACGATATCTCTAGCTTAGAGCTATAGAGATTAC
TCTACTGTGATGAAACTAAAGTAGTTTGCATTTAAGTCAAAATGCCAAATCAATATCATT
GTTTATTGAAAGCTGTTTCAGATTGTAAATGACTAGATGTGTTGGNTGTGTGCT

Last Updated on 06.12.2018 16:11