Sequences from confirmation amplicon


Confirmation sequences for line SALK_147747c and hit At3g57220 are shown.
Line ID SALK_147747c
Locus At3g57220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21174316
Primers used for PCR 07uk - R212, show primer details
   
Sequence Name 26-K117581-SALK_147747C-07uk
GenBank Accession LN485044 [GenBank]
Sequencing primer gene specific primer, 07uk: 5'-GGAATCAACTTGAGTGTGACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117581-SALK_147747C-07uk
AAACAGAGAAGGTAAAGATAAAGCGAACACAGAAGCGAAGAGATCACGAAAATCACGCCC
AATTTCGGCGGCGCGAGCTGGAAATCCTCTCCGGAAACAGAGGTCTTCTGAACCGTCGGG
GAAGATGCAGGTTCAGGTAGAGCTAGTTTCTCCGAATTGGACACTGAAGGAGTTCTCTTA
CGAGCAGTCATGGCGGATCCGTTGGCTATTTCTGGTTCCCCGCCTCTGGATTTGCTTCTT
CCACTTTTCGCTGAAGCTTCAAAGCCTGTAGCATTTAATTACAACTTGCCCACGCACAAC
AATACGACATCGTATCAACACCACTTATTTATGAATTATCAAAAAGGCCCAAAAAAGCAA
AGTATAAAGAAATAAATAAGTCTATGGGATTGACGGGAAACGACAATCTGATCATGAGCG
GAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGT
TTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTCTGGAGTTTA
ATGAGCTAAGCACATACGTCAGAAACCATTATTGCGCGTTCA
   
Sequence Name 10-K117580-SALK_147747C-R214
GenBank Accession LN484566 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117580-SALK_147747C-R214
GGCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCG
TCAATCCCATAGACTTATTTATTTCTTTATACTTTGCTTTTTTGGGCCTTTTTGATAATT
CATAAATAAGTGGTGTTGATACGATGTCGTATTGTTGTGCGTGGGCAAGTTGTAATTAAA
TGCTACAGGCTTTGAAGCTTCAGCGAAAAGTGGAAGAAGCAAATCCAGAGGCGGGGAACC
AGAAATAGCCAACGGATCCGCCATGACTGCTCGTAAGAGAACTCCTTCAGTGTCCAATTC
GGAGAAACTAGCTCTACCTGAACCTGCATCTTCCCCGACGGTTCAGAAGACCTCTGTTTC
CGGAGAGGATTTCCAGCTCGCGCCGCCGAAATTGGGCGTGATTTTCGTGATCTCTTCGCT
TCTGTGTTCGCTTTATCTTTACCTTCTCTGTTTCCACTACAATGTTGACAATGAGCTCAA
GCGTCCGATCCTCATTAACGCTGGGCTTAGCTTGGTTGGATTCTTTGTCACACTCA

Last Updated on 06.12.2018 16:11